Ambrus J L, Jurgensen C H, Brown E J, Fauci A S
J Exp Med. 1985 Oct 1;162(4):1319-35. doi: 10.1084/jem.162.4.1319.
High molecular weight B cell growth factor (HMW-BCGF) produced by a T cell line was purified to homogeneity and demonstrated to bind specifically to activated human B cells. A monoclonal antibody to HMW-BCGF was developed that (a) specifically inhibited the activity of HMW-BCGF in enhancing B cell proliferation, (b) specifically bound to HMW-BCGF in Western blots, (c) specifically absorbed HMW-BCGF activity from culture supernatants, and (d) specifically absorbed an internally labeled protein from T-ALL supernatant which comigrates with HMW-BCGF on sodium dodecyl sulfate-polyacrylamide gels. This antibody should help in cloning the gene for HMW-BCGF and further exploring the physiologic roles of HMW-BCGF.
一种T细胞系产生的高分子量B细胞生长因子(HMW-BCGF)被纯化至均一,并证明其能特异性结合活化的人B细胞。针对HMW-BCGF开发了一种单克隆抗体,该抗体:(a)能特异性抑制HMW-BCGF增强B细胞增殖的活性;(b)在蛋白质免疫印迹中能特异性结合HMW-BCGF;(c)能从培养上清液中特异性吸附HMW-BCGF的活性;(d)能从T-ALL上清液中特异性吸附一种在十二烷基硫酸钠-聚丙烯酰胺凝胶上与HMW-BCGF迁移率相同的内部标记蛋白。这种抗体应有助于克隆HMW-BCGF的基因,并进一步探索HMW-BCGF的生理作用。