Butler J L, Falkoff R J, Fauci A S
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2475-8. doi: 10.1073/pnas.81.8.2475.
A cloned human T-cell hybridoma (7D5) secreting B-cell growth factor (BCGF) and B-cell differentiation factor (BCDF) was established. Supernatant from this hybrid was capable of maintaining proliferation in anti-IgM-activated normal human B cells. In addition, the hybridoma supernatant induced differentiation and antibody secretion in Staphylococcus aureus Cowan I-stimulated B cells. No interleukin 2 was present in supernatant from this hybridoma. Molecular size of the hybridoma-derived BCGF and BCDF was determined by gel filtration chromatography. BCGF activity was present in the 20-kDa fractions, and BCDF activity eluted in the 30- to 35-kDa fractions. The isoelectric points of the factors, determined by chromatofocusing, were 6.6 for BCGF and 5.9 for BCDF. Finally, absorption experiments were performed using specific target cells. Phytohemagglutinin-stimulated T-cell blasts did not remove either BCGF or BCDF activity. Anti-IgM-activated B cells absorbed BCGF but not BCDF. In contrast, CESS cells removed BCDF but not BCGF. Thus, a human T-cell hybridoma secreting two distinct B-cell lymphokines was developed. Further immunochemical and functional studies of these immunoregulatory molecules should greatly enhance our understanding of the regulation of human B-cell function in normal and disease states.
建立了一株克隆的人T细胞杂交瘤（7D5），它能分泌B细胞生长因子（BCGF）和B细胞分化因子（BCDF）。该杂交瘤的上清液能够维持抗IgM激活的正常人B细胞的增殖。此外，杂交瘤上清液能诱导金黄色葡萄球菌Cowan I刺激的B细胞分化并分泌抗体。该杂交瘤的上清液中不存在白细胞介素2。通过凝胶过滤色谱法测定了杂交瘤来源的BCGF和BCDF的分子大小。BCGF活性存在于20 kDa的组分中，BCDF活性在30至35 kDa的组分中洗脱。通过色谱聚焦法测定，这些因子的等电点分别为：BCGF为6.6，BCDF为5.9。最后，使用特定靶细胞进行了吸收实验。植物血凝素刺激的T细胞母细胞不能去除BCGF或BCDF的活性。抗IgM激活的B细胞能吸收BCGF但不能吸收BCDF。相反，CESS细胞能去除BCDF但不能去除BCGF。因此，开发出了一种能分泌两种不同B细胞淋巴因子的人T细胞杂交瘤。对这些免疫调节分子进行进一步的免疫化学和功能研究，应能极大地增进我们对正常和疾病状态下人B细胞功能调节的理解。
