Leng Maodong, Jiang Huiqin, Zhang Sitong, Bao Yixi
Department of Clinical Laboratory, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Department of Clinical Laboratory, Zhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450018, China.
ACS Omega. 2024 May 2;9(19):21144-21151. doi: 10.1021/acsomega.4c01025. eCollection 2024 May 14.
Hepatic carcinoma is one of the leading causes of morbidity and mortality among all cancers, but no effective treatment measures have been developed. Herein, polystyrene polysaccharide (PSP) extracted from was used to synthesize gold nanoparticles (PSP-AuNPs) by heating and reduction methods, and the characteristics of the PSP-AuNPs were detected after successful synthesis. , the immunoregulatory effects of PSP-AuNPs were studied by testing the concentrations of NO, TNF-α, and IL-12p70 in the culture media of PSP-AuNPs-treated RAW264.7 macrophages, and the effect of biocompatibility on the viability of RAW264.7 macrophages and L02 cells was studied a CCK-8 assay. , tumor-bearing mice were established and treated with PSP-AuNPs, and the anticancer effects were studied by detecting trends in tumor volume, tumor inhibition rate, and tumor cell proliferation index. Immunoregulation was assessed by evaluating the serum levels of TNF-α and IL-10, the CD4+/CD8+ lymphocyte ratio in peripheral blood and the spleen and thymus indices; toxicity was investigated by measuring body weight, liver and renal function indices. The results showed that PSP-AuNPs could regulate immune function both and with almost no toxicity. PSP-AuNPs exhibited excellent anticancer effects on hepatic carcinoma . The anticancer effect could be strengthened, and the toxicity could be reduced by the combined use of PSP-AuNPs and ADM. In conclusion, PSP-AuNPs could be effective as a therapy and adjuvant therapy for treating hepatic carcinoma, providing potential treatment strategies for this disease.
肝癌是所有癌症中导致发病和死亡的主要原因之一，但尚未开发出有效的治疗措施。在此，从[未提及具体来源]中提取的聚苯乙烯多糖（PSP）通过加热和还原方法用于合成金纳米颗粒（PSP-AuNPs），成功合成后检测了PSP-AuNPs的特性。此外，通过检测PSP-AuNPs处理的RAW264.7巨噬细胞培养基中NO、TNF-α和IL-12p70的浓度，研究了PSP-AuNPs的免疫调节作用，并通过CCK-8测定法研究了生物相容性对RAW264.7巨噬细胞和L02细胞活力的影响。另外，建立了荷瘤小鼠并用PSP-AuNPs进行治疗，通过检测肿瘤体积、肿瘤抑制率和肿瘤细胞增殖指数的变化趋势来研究其抗癌效果。通过评估血清中TNF-α和IL-10的水平、外周血以及脾脏和胸腺中CD4+/CD8+淋巴细胞比率来评估免疫调节；通过测量体重、肝肾功能指标来研究毒性。结果表明，PSP-AuNPs在体内和体外均可调节免疫功能且几乎无毒性。PSP-AuNPs对肝癌表现出优异的抗癌效果。PSP-AuNPs与阿霉素联合使用可增强抗癌效果并降低毒性。总之，PSP-AuNPs可作为治疗肝癌的有效治疗方法和辅助治疗方法，为该疾病提供了潜在的治疗策略。
