Zhang Han, Yang Meiqing, Zhang Hongling, Ren Zheng, Wang Qiyan, Liu Yubo, Jin Xiaoye, Ji Jingyan, Feng Yuhang, Cai Changsheng, Ran Qianchong, Li Chengtao, Huang Jiang
Department of Forensic Medicine, Guizhou Medical University, Guiyang, Guizhou, China.
Institute of Forensic Science, Fudan University, Shanghai, China.
Forensic Sci Res. 2024 Jan 16;9(2):owad052. doi: 10.1093/fsr/owad052. eCollection 2024 Jun.
Insertion/Deletion (InDel) polymorphisms, characterized by their smaller amplicons, reduced mutation rates, and compatibility with the prevalent capillary electrophoresis (CE) platforms in forensic laboratories, significantly contribute to the advancement and application of genetic analysis. Guizhou province in China serves as an important region for investigating the genetic structure, ethnic group origins, and human evolution. However, DNA data and the sampling of present-day populations are lacking, especially about the InDel markers. Here, we reported data on 47 autosomal InDels from 592 individuals from four populations in Guizhou (Han, Dong, Yi, and Chuanqing). Genotyping was performed with the AGCU InDel 50 kit to evaluate their utility for forensic purposes and to explore the population genetic structure. Our findings showed no significant deviations from Hardy-Weinberg and linkage equilibriums. The combined power of discrimination (CPD) and the combined power of exclusion (CPE) for each population demonstrated that the kit could be applied to forensic individual identification and was an effective supplement for parentage testing. Genetic structure analyses, including principal component analysis, multidimensional scaling, genetic distance calculation, STRUCTURE, and phylogenetic analysis, highlighted that the genetic proximity of the studied populations correlates with linguistic, geographical, and cultural factors. The observed genetic variances within four research populations were less pronounced than those discerned between populations across different regions. Notably, the Guizhou Han, Dong, and Chuanqing populations showed closer genetic affiliations with linguistically similar groups than the Guizhou Yi. These results underscore the potential of InDel markers in forensic science and provide insights into the genetic landscape and human evolution in multi-ethnic regions like Guizhou.
InDel markers show promise for forensic individual identification and parentage testing via the AGCU InDel 50 kit.Genetic analysis of Guizhou populations reveals correlations with linguistic, geographical, and cultural factors.Guizhou Han, Dong, and Chuanqing populations showed closer genetic affiliations with linguistically similar groups than the Guizhou Yi.
插入/缺失（InDel）多态性，其特点是扩增子较小、突变率降低且与法医实验室中普遍使用的毛细管电泳（CE）平台兼容，对基因分析的发展和应用有重大贡献。中国贵州省是研究遗传结构、族群起源和人类进化的重要地区。然而，目前缺乏DNA数据以及现代人群的样本，尤其是关于InDel标记的数据。在此，我们报告了来自贵州四个群体（汉族、侗族、彝族和穿青人）的592名个体的47个常染色体InDel的数据。使用AGCU InDel 50试剂盒进行基因分型，以评估其在法医鉴定中的效用并探索群体遗传结构。我们的研究结果表明，这些数据与哈迪-温伯格平衡和连锁平衡无显著偏差。每个群体的联合鉴别力（CPD）和联合排除力（CPE）表明，该试剂盒可用于法医个体识别，是亲子鉴定的有效补充。遗传结构分析，包括主成分分析、多维尺度分析、遗传距离计算、STRUCTURE分析和系统发育分析，突出表明所研究群体的遗传相似性与语言、地理和文化因素相关。在四个研究群体中观察到的遗传差异不如在不同地区群体之间观察到的差异明显。值得注意的是，贵州汉族、侗族和穿青人群体与语言相似群体的遗传关系比贵州彝族更密切。这些结果强调了InDel标记在法医学中的潜力，并为贵州等多民族地区的遗传景观和人类进化提供了见解。
InDel标记通过AGCU InDel 50试剂盒在法医个体识别和亲子鉴定方面显示出前景。对贵州群体的遗传分析揭示了与语言、地理和文化因素的相关性。贵州汉族、侗族和穿青人群体与语言相似群体的遗传关系比贵州彝族更密切。
