Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, China.
School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China.
Mol Genet Genomics. 2020 Jul;295(4):969-979. doi: 10.1007/s00438-020-01673-x. Epub 2020 Apr 11.
The Tibetan-Yi Corridor, located on the eastern edge of the Tibetan Plateau, is the main route of the people of the plateau. Human settlement and diffusion along the corridor have played a pivotal role in shaping the genetic architecture of Sino-Tibetan-speaking (STs) populations in China. In this study, five STs groups (Chengdu Tibetan, Chengdu Han, Muli Tibetan, Lugu Lake Mosuo and Xichang Yi) settling in the Tibetan-Yi Corridor were genotyped via AGCU InDel 50 kit on the capillary electrophoresis platform to decrypt the genetic landscape and phylogenetic relationship of STs populations and investigate the forensic characteristics. Allele frequency distributions of all autosomal insertion/deletion polymorphisms (InDels) in studied groups comply with Hardy-Weinberg equilibrium. The combined power of discrimination values are 0.9999999999999999998, 0.9999999999999999995, 0.9999999999999999999, 0.999999999999999993 and 0.99999999999999999994, respectively, and all the combined probability of exclusion values exceed 0.9990. Forensically relevant statistics implied that these InDels could be used for individual identification and as a promising alternative to STR profiling in paternity testing. Typical population comparisons showed strikingly high homogeneity among studied STs people, indicating complicated genetic admixture among populations in the Tibetan-Yi Corridor. The STs groups in the Tibetan-Yi Corridor keep close genetic affinity with geographically or linguistically close populations, and the genetic components of investigated populations arose from a mixture of multiple ancestral gene pools (resulting from the admixture from the ancestral Highland Tibetans and ancestral Lowland indigenous populations).
藏彝走廊位于青藏高原的东缘，是高原人民的主要通道。走廊沿线的人类定居和扩散在塑造中国藏缅语族群的遗传结构方面发挥了关键作用。在这项研究中，对居住在藏彝走廊的五个藏缅语族群（成都藏族、成都汉族、木里藏族、泸沽湖摩梭人和西昌彝族）进行了 AGCU InDel 50 试剂盒的毛细管电泳平台基因分型，以解密藏缅语族群的遗传景观和系统发育关系，并调查法医特征。所有常染色体插入/缺失多态性（InDels）的等位基因频率分布在研究群体中均符合 Hardy-Weinberg 平衡。组合鉴别力值分别为 0.9999999999999999998、0.9999999999999999995、0.9999999999999999999、0.999999999999999993 和 0.99999999999999999994，所有排除值的组合概率均超过 0.9990。法医学相关统计数据表明，这些 InDels 可用于个体识别，是 STR 谱分析在亲子鉴定中的一种很有前途的替代方法。典型的群体比较表明，研究中的藏缅语族群之间具有极高的同质性，表明藏彝走廊人群之间存在复杂的遗传混合。藏彝走廊的藏缅语族群与地理上或语言上相近的族群保持着密切的遗传亲和力，调查人群的遗传成分来自多个祖先基因库的混合（由远古高地藏族人和远古低地土著人口的混合而产生）。
