大鼠下颌下腺提取物中的因子对体外淋巴细胞反应性的抑制和增强作用。
Suppression and enhancement of in vitro lymphocyte reactivity by factors in rat submandibular gland extracts.
作者信息
Kemp A, Mellow L, Sabbadini E
出版信息
Immunology. 1985 Oct;56(2):261-7.
The addition of crude extracts from rat submandibular (SM) glands to murine spleen and lymph node cell cultures stimulated with concanavalin A (Con A) induced either suppression (at high concentrations) or further stimulation (at lower concentrations) or further stimulation (at lower concentrations) of proliferative activity. Gel filtration of the extracts revealed that suppressive activity was due to factors of molecular weight in the 50,000-96,000 range, while stimulation was due to factors in the 13,000-35,000 molecular weight range. The suppressor activity of the higher molecular weight fractions was not due to a reduction of cell viability or of the uptake of tritiated thymidine. This was demonstrated by the fact that the addition of IL-2 to the cultures completely reversed the suppressive effect. Further fractionation of the suppressive and of the stimulatory gel filtration fractions with the chromatofocusing technique led to the identification of a single fraction with suppressor activity and of multiple discrete fractions with stimulatory activity.
将大鼠颌下腺粗提物添加到用伴刀豆球蛋白A（Con A）刺激的小鼠脾脏和淋巴结细胞培养物中，可诱导增殖活性的抑制（高浓度时）或进一步刺激（低浓度时）。提取物的凝胶过滤显示，抑制活性归因于分子量在50,000 - 96,000范围内的因子，而刺激则归因于分子量在13,000 - 35,000范围内的因子。较高分子量级分的抑制活性并非由于细胞活力降低或氚标记胸腺嘧啶摄取减少所致。这一事实表明，向培养物中添加白细胞介素-2可完全逆转抑制作用。用色谱聚焦技术对抑制性和刺激性凝胶过滤级分进行进一步分级分离，从而鉴定出具有抑制活性的单一级分和具有刺激活性的多个离散级分。
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