Nanhu Laboratory, National Center of Biomedical Analysis, Beijing, China.
Nanhu Laboratory, National Center of Biomedical Analysis, Beijing, China; School of Medicine, Tsinghua University, Beijing, China.
Cell. 2024 Jun 6;187(12):2935-2951.e19. doi: 10.1016/j.cell.2024.04.027. Epub 2024 May 20.
Peripheral sensory neurons widely innervate various tissues to continuously monitor and respond to environmental stimuli. Whether peripheral sensory neurons innervate the spleen and modulate splenic immune response remains poorly defined. Here, we demonstrate that nociceptive sensory nerve fibers extensively innervate the spleen along blood vessels and reach B cell zones. The spleen-innervating nociceptors predominantly originate from left T8-T13 dorsal root ganglia (DRGs), promoting the splenic germinal center (GC) response and humoral immunity. Nociceptors can be activated by antigen-induced accumulation of splenic prostaglandin E (PGE) and then release calcitonin gene-related peptide (CGRP), which further promotes the splenic GC response at the early stage. Mechanistically, CGRP directly acts on B cells through its receptor CALCRL-RAMP1 via the cyclic AMP (cAMP) signaling pathway. Activating nociceptors by ingesting capsaicin enhances the splenic GC response and anti-influenza immunity. Collectively, our study establishes a specific DRG-spleen sensory neural connection that promotes humoral immunity, suggesting a promising approach for improving host defense by targeting the nociceptive nervous system.
周围感觉神经元广泛支配各种组织，以持续监测和响应环境刺激。周围感觉神经元是否支配脾脏并调节脾脏免疫反应尚不清楚。在这里，我们证明伤害感受性感觉神经纤维广泛地沿着血管支配脾脏，并到达 B 细胞区。支配脾脏的伤害感受器主要来源于左侧 T8-T13 背根神经节（DRG），促进脾脏生发中心（GC）反应和体液免疫。伤害感受器可被抗原诱导的脾脏前列腺素 E（PGE）积累激活，然后释放降钙素基因相关肽（CGRP），进一步促进早期的脾脏 GC 反应。在机制上，CGRP 通过其受体 CALCRL-RAMP1 通过环磷酸腺苷（cAMP）信号通路直接作用于 B 细胞。通过摄入辣椒素来激活伤害感受器可增强脾脏 GC 反应和抗流感免疫力。总之，我们的研究建立了一个特定的 DRG-脾脏感觉神经连接，促进体液免疫，提示通过靶向伤害性神经系统来提高宿主防御的有前途的方法。
