National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaiyin Institute of Technology, Huaian, 223003, PR China.
National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaiyin Institute of Technology, Huaian, 223003, PR China.
Anal Chim Acta. 2024 Jun 22;1309:342701. doi: 10.1016/j.aca.2024.342701. Epub 2024 May 7.
BACKGROUND: Nanozymes, a new class of nanomaterials, have emerged as promising substitutes for enzymes in biosensor design due to their exceptional stability, affordability, and ready availability. While nanozymes address many limitations of natural enzymes, they still face challenges, particularly in achieving the catalytic activity levels of their natural counterparts. This indicates the need for enhancing the sensitivity of biosensors based on nanozymes. The catalytic activity of nanozyme can be significantly improved by regulating its size, morphology, and surface composition of nanomaterial. RESULTS: In this work, a kind of hollow core-shell structure was designed to enhance the catalytic activity of nanozymes. The hollow core-shell structure material consists of a nanozymes core layer, a hollow layer, and a MOF shell layer. Taking the classic peroxidase like FeO as an example, the development of a novel nanozyme@MOF, specifically p-FeO@PDA@ZIF-67, is detailed, showcasing its application in enhancing the sensitivity of sensors based on FeO nanozymes. This innovative nanocomposite, featuring that MOF layer was designed to adsorb the signal molecules of the sensor to improve the utilization rate of reactive oxygen species generated by the nanozymes catalyzed reactions and the hollow layer was designed to prevent the active sites of nanozymes from being cover by the MOF layer. The manuscript emphasizes the nanocomposite's remarkable sensitivity in detecting hydrogen peroxide (HO), coupled with high specificity and reproducibility, even in complex environments like milk samples. SIGNIFICANCE AND NOVELTY: This work firstly proposed and proved that FeO nanozyme@MOF with hollow layer structure was designed to improve the catalytic activity of the FeO nanozyme and the sensitivity of the sensors based on FeO nanozyme. This research marks a significant advancement in nanozyme technology, demonstrating the potential of structural innovation in creating high-performance, sensitive, and stable biosensors for various applications.
背景：纳米酶作为一类新型纳米材料，由于其出色的稳定性、经济性和易得性，已成为生物传感器设计中酶的有前途的替代品。虽然纳米酶解决了许多天然酶的局限性，但它们仍然面临挑战，特别是在达到天然酶的催化活性水平方面。这表明需要提高基于纳米酶的生物传感器的灵敏度。通过调节纳米材料的尺寸、形态和表面组成，可以显著提高纳米酶的催化活性。
结果：在这项工作中，设计了一种空心核壳结构来提高纳米酶的催化活性。空心核壳结构材料由纳米酶核层、空心层和 MOF 壳层组成。以经典的过氧化物酶样 FeO 为例，详细介绍了一种新型纳米酶@MOF 的开发，即 p-FeO@PDA@ZIF-67，展示了其在提高基于 FeO 纳米酶的传感器灵敏度方面的应用。这种创新的纳米复合材料，MOF 层设计用于吸附传感器的信号分子，以提高纳米酶催化反应生成的活性氧的利用率，空心层设计用于防止 MOF 层覆盖纳米酶的活性位点。本文强调了该纳米复合材料在检测过氧化氢（HO）方面的卓越灵敏度，同时具有高特异性和重现性，即使在复杂环境如牛奶样品中也是如此。
意义和新颖性：本工作首次提出并证明了具有空心层结构的 FeO 纳米酶@MOF 被设计用来提高 FeO 纳米酶的催化活性和基于 FeO 纳米酶的传感器的灵敏度。这项研究标志着纳米酶技术的重大进展，展示了结构创新在为各种应用创造高性能、敏感和稳定的生物传感器方面的潜力。
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