Nanozymes have garnered considerable research interest for their unique capacity to bridge nanotechnology and biology. Current studies predominantly concentrate on exploring nanozymes with diverse catalytic activities and their potential applications across various disciplines. Among them, nanoscale metal-organic frameworks (MOFs) are promising nanomaterials for constructing nanozymes. In this review, we firstly introduce the general construction strategies for MOF-based nanozymes. In addition, we also classify the MOF-based nanozymes in detail based on their catalytic performance. Thirdly, the recent research progress of MOF-based nanozymes in the field of biosensing, cancer therapy, antibacterial infection, and antioxidation are also comprehensively reviewed. Finally, we discuss the current challenges and future perspectives of MOF-based nanozymes, with the aim of assisting in their construction and maximizing their potential in bioapplications. It is hoped that we could provide scientists in materials science and biomedical research with valuable and comprehensive information, fostering advancements in interdisciplinary fields.