在质粒R100 - 1的r - 决定子mer区域中发生的缺失是为了选择丧失汞超敏性的菌株而进行的。
Deletions in the r-determinant mer region of plasmid R100-1 selected for loss of mercury hypersensitivy.
作者信息
Foster T J, Nakahara H
出版信息
J Bacteriol. 1979 Oct;140(1):301-5. doi: 10.1128/jb.140.1.301-305.1979.
Abstract
A mutant of plasmid R100-1, which conferred cellular hypersensitivity to Hg2+ because of the insertion of Tn801 (TnA) into the gene determining synthesis of mercuric reductase enzyme, allowed further mutational events to be selected which resulted in either reversion to Hg2+ resistance (characteristic plasmid R100-1) or sensitivity at a level characteristic of plasmidless strains. Restriction endonuclease EcoRI and BamHI analysis showed that reversion to resistance resulted from loss of TnA from the R100-mer:Tn801 plasmid, whereas the change from hypersensitivity to sensitivity to Hg2+ usually resulted from deletion of part or all of Tn801 plus plasmid deoxyribonucleic acid sequences corresponding to the operator-proximal end of the mer operon.
摘要
质粒R100 - 1的一个突变体，由于Tn801（TnA）插入到决定汞还原酶合成的基因中，导致细胞对Hg2+超敏，该突变体使得进一步的突变事件得以被选择出来，这些突变事件要么导致回复到Hg2+抗性（典型的质粒R100 - 1），要么导致达到无质粒菌株特征水平的敏感性。限制性内切酶EcoRI和BamHI分析表明，抗性回复是由于R100 - mer:Tn801质粒上TnA的缺失，而从对Hg2+超敏到敏感的变化通常是由于部分或全部Tn801以及与mer操纵子操纵基因近端相对应的质粒脱氧核糖核酸序列的缺失所致。
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