The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, 550025, China.
College of Tea Sciences, Guizhou University, Guiyang, 550025, China.
Sci Rep. 2024 May 21;14(1):11603. doi: 10.1038/s41598-024-62319-1.
Zikui (Camellia sinensis cv. Zikui) is a recently discovered cultivar of local purple tea in Guizhou, China. It is a purple leaf bud mutation material of Meitan Taicha (Camellia sinensis cv. 'Meitan-taicha') 'N61' strain, which is an important local germplasm resource in Guizhou. It is also a model plant for the study of anthocyanins, but the limited germplasm resources and the limitation of traditional reproduction hinder its application. Here, an efficient regeneration system is established by using hypocotyl as explants for the first time. Different plant growth regulators (PGRs) are evaluated during different regeneration processes including callus and root induction. According to our findings, using the optimal disinfection conditions, the seed embryo contamination rate is 17.58%. Additionally, the mortality rate is 9.69%, while the survival rate is measured as 72.73%. Moreover, the highest germination rate of 93.64% is observed under MS + 2.40 mg/L GA medium conditions. The optimal callus induction rate is 95.19%, while the optimal adventitious bud differentiation rate is 20.74%, Medium with 1.6 mg/L IBA achieved 68.6% rooting of the adventitious shoots. The survival rate is more than 65% after 6 days growth in the cultivated matrix. In summary, our research aims to establish a regeneration system for Zikui tea plants and design a transformation system for tea plant tissue seedlings. This will enable transfer of the target gene and ultimately facilitate the cultivation of new tea varieties with unique characteristics.
紫魁（Camellia sinensis cv. Zikui）是中国贵州新发现的一种地方紫茶品种。它是梅潭台茶（Camellia sinensis cv. 'Meitan-taicha'）'N61'品系的一个紫色芽叶突变体材料，是贵州重要的地方种质资源。它也是花青素研究的模式植物，但有限的种质资源和传统繁殖的限制阻碍了它的应用。本研究首次以子叶为外植体，建立了高效的再生体系，评估了不同的植物生长调节剂（PGRs）在愈伤组织和生根诱导的不同再生过程中的作用。根据我们的发现，采用最优消毒条件，种子胚的污染率为 17.58%，死亡率为 9.69%，存活率为 72.73%。此外，在 MS+2.40mg/L GA 培养基中观察到最高的发芽率为 93.64%。最佳愈伤组织诱导率为 95.19%，最佳不定芽分化率为 20.74%，1.6mg/L IBA 培养基的不定芽生根率为 68.6%。在培养基质中生长 6 天后，成活率超过 65%。总之，本研究旨在建立紫魁茶树再生体系，设计茶树组织苗的转化体系，实现目的基因的转移，最终促进具有独特特性的新茶品种的培育。
