Neveu P J, Perdoux D
Int Arch Allergy Appl Immunol. 1985;78(4):401-5. doi: 10.1159/000233921.
The incorporation of 3H-thymidine in newly formed DNA was studied in guinea pig spleen cells stimulated by phytohemagglutinin (PHA) and/or concanavalin A (Con A). In spleen cells stimulated by PHA, two peaks of thymidine uptake were observed for two different doses of lectin whatever the number of cultured cells. Furthermore, thymidine uptake is proportional to the number of the cultured cells. During Con A-induced mitogenesis, two peaks were also observed but only when using a great concentration of cells. Maximal thymidine incorporation depended on both the cell number and the concentration of Con A. When cells were stimulated by both PHA and Con A, thymidine uptake was strongly depressed as compared to the one observed using PHA or Con A alone. On the other hand, supernatants from unstimulated spleen cells had opposite effects on blastogenesis induced by Con A or by PHA; they depressed PHA-induced blastogenesis while enhancing the one induced by Con A. These results suggest that, in guinea pigs, both PHA and Con A induce thymidine incorporation in at least two lymphocyte populations through different mechanisms. This heterogeneity of lectin-induced T cell mitogenesis has to be taken in account when studying the mechanisms by which the immunomodulators are active at the T cell level.
在由植物血凝素（PHA）和/或刀豆球蛋白A（Con A）刺激的豚鼠脾细胞中，研究了3H-胸腺嘧啶核苷掺入新形成DNA的情况。在由PHA刺激的脾细胞中，无论培养细胞的数量如何，对于两种不同剂量的凝集素都观察到两个胸腺嘧啶核苷摄取峰。此外，胸腺嘧啶核苷摄取与培养细胞的数量成正比。在Con A诱导的有丝分裂过程中，也观察到两个峰，但仅在使用高浓度细胞时出现。最大胸腺嘧啶核苷掺入量取决于细胞数量和Con A的浓度。当细胞同时受到PHA和Con A刺激时，与单独使用PHA或Con A时相比，胸腺嘧啶核苷摄取明显降低。另一方面，未刺激的脾细胞的上清液对Con A或PHA诱导的细胞增殖有相反的作用；它们抑制PHA诱导的细胞增殖，同时增强Con A诱导的细胞增殖。这些结果表明，在豚鼠中，PHA和Con A均通过不同机制在至少两个淋巴细胞群体中诱导胸腺嘧啶核苷掺入。在研究免疫调节剂在T细胞水平发挥作用的机制时，必须考虑凝集素诱导的T细胞有丝分裂的这种异质性。
