Söder O, Hellström P M
Pediatric Endocrinology Unit, Karolinska Hospital, Stockholm, Sweden.
Int Arch Allergy Appl Immunol. 1987;84(2):205-11. doi: 10.1159/000234424.
The effect of 15 defined neuropeptides on the mitogenic activation of lymphocytes from human thymus, guinea pig lymph nodes and rat spleen was investigated. Lymphocytes were incubated in the absence or presence of polyclonal T and B cell activators together with increasing doses of the neuropeptides, and harvested at 48 h of culture after pulse-labeling with 3H-thymidine to assess the DNA synthesis. A dose-related stimulatory effect on the spontaneous 3H-thymidine incorporation of human thymocytes was obtained with methionine-enkephalin (met-enk), motilin and neurotensin. Vasoactive intestinal polypeptide (VIP) and peptide HI (PHI) were inhibitory. A similar responsiveness was observed in cultures of phytohemagglutinin P (PHA)-activated human thymocytes. The low level of basal DNA synthesis of guinea pig lymph node cells was stimulated by VIP and inhibited by neuropeptide Y (NPY) and PHI. PHA-activated lymph node T lymphocytes were stimulated by neurotensin, bombesin and motilin, whereas NPY inhibited the thymidine uptake. The low rate of spontaneous DNA synthesis of rat spleen cells was increased in the presence of VIP. Met-enk stimulated both basal and dextran sulfate-activated splenic B cell proliferation, whereas PHI was inhibitory in both cases. The following peptides were found to be inactive in all the above assays: substance P, cholecystokinin-octapeptide, somatostatin, galanin, oxytocin, pentagastrin and gastrin-releasing peptide 1-27 and 14-27. Although the responses were generally of low magnitude and observed at high peptide concentrations, present study contributes to the understanding of possible mechanisms involved in interactions between the nervous and the immune system.
研究了15种特定神经肽对人胸腺、豚鼠淋巴结和大鼠脾脏淋巴细胞有丝分裂活化的影响。淋巴细胞在无或有多克隆T细胞和B细胞激活剂存在的情况下,与递增剂量的神经肽一起孵育,并在用3H-胸腺嘧啶核苷脉冲标记后于培养48小时收获,以评估DNA合成。甲硫氨酸脑啡肽(met-enk)、胃动素和神经降压素对人胸腺细胞自发的3H-胸腺嘧啶核苷掺入有剂量相关的刺激作用。血管活性肠肽(VIP)和肽HI(PHI)具有抑制作用。在植物血凝素P(PHA)激活的人胸腺细胞培养物中观察到类似的反应性。VIP刺激豚鼠淋巴结细胞低水平的基础DNA合成,而神经肽Y(NPY)和PHI则抑制该合成。神经降压素、蛙皮素和胃动素刺激PHA激活的淋巴结T淋巴细胞,而NPY抑制胸腺嘧啶核苷摄取。在VIP存在的情况下,大鼠脾细胞自发DNA合成的低速率增加。Met-enk刺激基础和硫酸葡聚糖激活的脾B细胞增殖,而PHI在两种情况下均具有抑制作用。发现以下肽在上述所有试验中均无活性:P物质、八肽胆囊收缩素、生长抑素、甘丙肽、催产素、五肽胃泌素以及胃泌素释放肽1-27和14-27。尽管反应通常幅度较小且在高肽浓度下观察到,但本研究有助于理解神经和免疫系统之间相互作用的可能机制。