Laboratory of Bioressources, Biotechnology, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, Mohammed the First University, 60000 Oujda, Morocco.
Laboratory of Anthropogenetic, Biotechnology and Health, Team of Nutritional Physiopathology, Neurosciences and Toxicology, Faculty of Sciences, Chouaib Doukkali University, 24000 El Jadida, Morocco.
J Smooth Muscle Res. 2024;60:10-22. doi: 10.1540/jsmr.60.10.
Functional bowel disorders (FBD) have a major potential to degrade the standards of public life. Juniperus oxycedrus L. (J. oxycedrus) (Cupressaceae) has been described as a plant used in traditional medicine as an antidiarrheal medication. The present study is the first to obtain information on the antispasmodic and antidiarrheic effects of J. oxycedrus aqueous extract through in vitro and in vivo studies. An aqueous extract of J. oxycedrus (AEJO) was extracted by decoctioning air-dried aerial sections of the plant. Antispasmodic activity was tested in an isolated jejunum segment of rats exposed to cumulative doses of drogue extract. The antidiarrheic activity was tested using diarrhea caused by castor oil, a transit study of the small intestine, and castor oil-induced enteropooling assays in mice. In the jejunum of rats, the AEJO (0.1, 0.3 and 1 mg/ml) diminished the maximum tone induced by low K (25 mM), while it exhibited a weak inhibitory effect on high K (75 mM) with an IC=0.49 ± 0.01 mg/ml and IC=2.65 ± 0.16 mg/ml, respectively. In the contractions induced by CCh (10 M), AEJO diminished the maximum tone, similar to that induced by low K (25 mM). with an IC=0.45 ± 0.02 mg/ml. The inhibitory effect of AEJO on low K induced contractions was significantly diminished in the presence of glibenclamide (GB) (0.3 µM) and 4-aminopyrimidine (4-AP) (100 µM), with IC values of 1.84 ± 0.09 mg/ml. and 1.63 ± 0.16 mg/ml, respectively). The demonstrated inhibitory effect was similar to that produced by a non-competitive antagonist acting on cholinergic receptors and calcium channels. In castor oil-induced diarrhea in mice, AEJO (100, 200, and 400 mg/kg) caused an extension of the latency time, a reduced defecation frequency, and a decrease in the amount of wet feces compared to the untreated group (distilled water). Moreover, it showed a significant anti-motility effect and reduced the amount of fluid accumulated in the intestinal lumen at all tested doses. These findings support the conventional use of Juniperus oxycedrus L. as a remedy for gastrointestinal diseases.
功能性肠病（FBD）极大地降低了公众生活的标准。Juniperus oxycedrus L.（J. oxycedrus）（柏科）已被描述为一种在传统医学中用作抗腹泻药物的植物。本研究首次通过体外和体内研究获得了 Juniperus oxycedrus 水提物的抗痉挛和抗腹泻作用的信息。J. oxycedrus 水提物（AEJO）通过煎煮植物的风干地上部分提取。通过在暴露于累积剂量药物提取物的分离空肠段中测试抗痉挛活性。通过蓖麻油引起的腹泻、小肠转运研究和蓖麻油诱导的肠池试验在小鼠中测试抗腹泻活性。在大鼠空肠中，AEJO（0.1、0.3 和 1mg/ml）减少了低 K（25mM）诱导的最大张力，而对高 K（75mM）表现出弱抑制作用，IC=0.49±0.01mg/ml 和 IC=2.65±0.16mg/ml。在 CCh（10μM）诱导的收缩中，AEJO 减少了最大张力，类似于低 K（25mM）诱导的张力。IC=0.45±0.02mg/ml。在存在格列本脲（GB）（0.3μM）和 4-氨基嘧啶（4-AP）（100μM）时，AEJO 对低 K 诱导的收缩的抑制作用显著减弱，IC 值分别为 1.84±0.09mg/ml 和 1.63±0.16mg/ml。这种抑制作用类似于作用于胆碱能受体和钙通道的非竞争性拮抗剂产生的抑制作用。在蓖麻油诱导的小鼠腹泻中，AEJO（100、200 和 400mg/kg）与未处理组（蒸馏水）相比，潜伏期延长、排便频率降低、湿粪便量减少。此外，它在所有测试剂量下均表现出显著的抗运动作用，并减少了肠腔中积聚的液体量。这些发现支持了 Juniperus oxycedrus L. 作为治疗胃肠道疾病的传统用途。
