Li Zimeng, Yuan Chan, Li Shunda, Zhang Yu, Bai Bin, Yang Fangping, Liu Pengpeng, Sang Wei, Ren Yong, Singh Ravi, Liao Pingan, Lan Caixia
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China.
Plant Dis. 2024 Dec;108(12):3550-3561. doi: 10.1094/PDIS-06-24-1195-RE. Epub 2024 Nov 15.
Stripe or yellow rust (YR) caused by () is an important foliar disease affecting wheat production globally. Resistant varieties are the most economically and environmentally effective way to manage this disease. The common winter wheat ( L.) cultivar Luomai 163 exhibited resistance to the races CYR32 and CYR33 at the seedling stage and showed a high level of adult plant resistance in the field. To understand the genetic basis of YR resistance in this cultivar, 142 F recombinant inbred lines (RILs) derived from cross Apav#1 × LM163 and both parents were genotyped with the 16K SNP array and bulked segregant analysis sequencing. The analysis detected a major gene, , at the seedling stage associated with the 1BL.1RS translocation. Additionally, three genes for resistance at the adult plant stage were detected on chromosome arms 1BL (), 6BS, and 6BL in Luomai 163, whereas Apav#1 contributed resistance at a quantitative trait locus (QTL) on 2BL. These QTL explained YR disease severity variations ranging from 6.9 to 54.8%. The kompetitive allele-specific PCR (KASP) markers , , and for the three novel loci , , and were developed and validated. , , and showed varying degrees of resistance to YR when present individually or in combination based on genotype and phenotype analysis of a panel of 570 wheat accessions. Six RILs combining resistance alleles of all QTL, showing higher resistance to YR in the field than Luomai 163 with disease severities of 10.7 to 16.0%, are important germplasm resources for breeding programs to develop YR-resistant wheat varieties with good agronomic traits.
由（）引起的条锈病或黄锈病（YR）是影响全球小麦生产的一种重要叶部病害。抗性品种是防治该病害最经济且环保的方法。普通冬小麦（L.）品种洛麦163在苗期对条锈菌生理小种CYR32和CYR33表现出抗性，且在田间表现出高水平的成株抗性。为了解该品种对条锈病抗性的遗传基础，对由Apav#1×LM163杂交产生的142个F重组自交系（RILs）及其双亲进行了16K SNP芯片基因分型和混合分组分析法测序。分析在苗期检测到一个与1BL.1RS易位相关的主效基因，。此外，在洛麦163的染色体臂1BL（）、6BS和6BL上检测到三个成株期抗性基因（），而Apav#1在2BL上的一个数量性状位点（QTL）贡献了抗性。这些QTL解释了条锈病病情严重程度6.9%至54.8%的变异。开发并验证了针对三个新位点、和的竞争性等位基因特异性PCR（KASP）标记、和。基于对570份小麦种质资源的基因型和表型分析，、和单独或组合存在时对条锈病表现出不同程度的抗性。六个组合了所有QTL抗性等位基因的RILs在田间对条锈病的抗性高于洛麦163，病情严重程度为10.7%至16.0%，是育种计划中用于培育具有良好农艺性状的抗条锈病小麦品种的重要种质资源。
