Agrinier Anne-Laure, Pilon Geneviève, Marette André
Department of Medicine, Faculty of Medicine, Québec Heart and Lung Institute (IUCPQ), Université Laval, Québec, QC, Canada.
Center for Nutrition, Health and Society (NUTRISS), Institute of Nutrition and Functional Foods (INAF), Université Laval Québec, Québec, QC, Canada.
Front Microbiol. 2024 May 8;15:1250366. doi: 10.3389/fmicb.2024.1250366. eCollection 2024.
gut models allow for the study of the impact of molecules on human gut microbiota composition and function without the implication of the host. However, current models, such as the Simulator of Human Intestinal Microbial Ecosystem (SHIME), are expensive, time-consuming, and require specialized personnel. Homemade models that lessen these issues have limited evidence of their humanlike functionality. In this study, we present the development of a low-cost and easy-to-use bioreactor with the proven functionality of human microbiota. In our model, we evaluated the capability of replicating human gut microbiota growth and the response of the human bacterial community to a prebiotic, resistant starch, particularly resistant starch type 2 (RS2). Our bioreactor produced an environment that was stable for pH, temperature, and anaerobic conditions. The bioreactor consistently cultivated bacterial communities over a 48 h time period, replicating the composition of the gut microbiota and the associated metabolite production response to RS2, in line with prior human studies. In response to the RS2 prebiotic, we observed an increase in and and an increase in the production of the short-chain fatty acids such as acetate, propionate, and isobutyrate. Taken together, these data demonstrate that our low-cost and user-friendly prototype bioreactor model provides a favorable environment for the growth of human gut microbiota and can mimic its response to a prebiotic.
肠道模型能够在不涉及宿主的情况下，研究分子对人类肠道微生物群组成和功能的影响。然而，目前的模型，如人类肠道微生物生态系统模拟器（SHIME），成本高昂、耗时且需要专业人员。能减少这些问题的自制模型，其类人功能的证据有限。在本研究中，我们展示了一种低成本且易于使用的生物反应器的开发，该反应器具有已被证实的人类微生物群功能。在我们的模型中，我们评估了复制人类肠道微生物群生长的能力以及人类细菌群落对益生元抗性淀粉，特别是2型抗性淀粉（RS2）的反应。我们的生物反应器产生了一个pH值、温度和厌氧条件稳定的环境。该生物反应器在48小时内持续培养细菌群落，复制了肠道微生物群的组成以及对RS2的相关代谢产物产生反应，这与先前的人体研究一致。针对RS2益生元，我们观察到 和 的增加以及乙酸盐、丙酸盐和异丁酸盐等短链脂肪酸产量的增加。综上所述，这些数据表明，我们的低成本且用户友好的原型生物反应器模型为人类肠道微生物群的生长提供了有利环境，并能模拟其对益生元的反应。
