Lee A K, Rosen P P, DeLellis R A, Saigo P E, Gangi M D, Groshen S, Bagin R, Wolfe H J
Am J Clin Pathol. 1985 Dec;84(6):687-96. doi: 10.1093/ajcp/84.6.687.
A collaborative immunohistochemical study was carried out to examine the expression and prognostic significance of tumor markers in a retrospective series of 233 invasive breast carcinomas. The patterns of tumor marker expression in 94 patients with short remission duration (recurrence within five years) were compared with 50 patients with intermediate (at five to ten years) and 89 patients with long (no recurrence at ten years or longer) remission durations. The antigens examined were carcinoembryonic antigen (CEA), human chorionic gonadotropin (HCG), placental lactogen, alpha-lactalbumin, and pregnancy-specific beta-1 glycoprotein. Carcino-embryonic antigen was the most frequently expressed antigen, whereas HCG was demonstrated least frequently. Also, the ABH isoantigen status was examined using monoclonal antibodies; isoantigen expression was observed in a subset of breast carcinomas, contrary to previous reports of total deletion in breast cancer. Two of the markers, CEA and HCG, were examined by both laboratories, each with two different antisera and also with both PAP and ABC immunohistochemical technics. Meticulous efforts were taken to provide quality control and ensure reproducibility of results. These included the use of serial sections of duplicate pathologic material by both institutions, standardization of experimental conditions and interpretation criteria, double-blind evaluation of exchanged slides, and use of standardized data sheets to record staining extent and intensity. No significant disagreements were observed between data obtained through the different approaches. The steps that were taken to minimize interobserver and interinstitutional differences in this study are presented as a model for collaborative immunohistochemical studies. The expression of tumor markers, alone or in combination, was not found to bear any significant relationship to prognostic indicators, such as the likelihood of recurrence, interval before recurrence, or presence of metastasis.
开展了一项联合免疫组织化学研究，以检测233例浸润性乳腺癌回顾性系列病例中肿瘤标志物的表达及其预后意义。将94例缓解期短（5年内复发）的患者、50例缓解期中度（5至10年）的患者和89例缓解期长（10年或更长时间无复发）的患者的肿瘤标志物表达模式进行了比较。检测的抗原包括癌胚抗原（CEA）、人绒毛膜促性腺激素（HCG）、胎盘催乳素、α-乳白蛋白和妊娠特异性β-1糖蛋白。癌胚抗原是表达最频繁的抗原，而HCG的表达最少。此外，使用单克隆抗体检测ABH同种抗原状态；与先前报道的乳腺癌中完全缺失相反，在一部分乳腺癌中观察到了同种抗原表达。两个实验室都用两种不同的抗血清以及PAP和ABC免疫组织化学技术检测了其中两种标志物CEA和HCG。为保证质量控制并确保结果的可重复性付出了精心努力。这些努力包括两个机构都使用重复病理材料的连续切片、实验条件和判读标准的标准化、交换切片的双盲评估以及使用标准化数据表记录染色范围和强度。通过不同方法获得的数据之间未观察到明显差异。本研究中为尽量减少观察者间和机构间差异所采取的步骤作为联合免疫组织化学研究的一个范例予以展示。未发现肿瘤标志物单独或联合表达与预后指标（如复发可能性、复发前间隔或转移情况）有任何显著关系。
