Lamichhane Jyoti, Choi Brian I, Stegman Natalie, Fontes Noronha Melline, Wolfe Alan J
Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153, USA.
Bioinformatics Program, Loyola University Chicago, Chicago, IL 60660, USA.
Antibiotics (Basel). 2024 May 10;13(5):433. doi: 10.3390/antibiotics13050433.
The recognition of the complex (AUC) as an emerging uropathogen has led to growing concerns due to a limited understanding of its disease spectrum and antibiotic resistance profiles. Here, we investigated the prevalence of macrolide resistance within urinary AUC isolates, shedding light on potential genetic mechanisms. Phenotypic testing revealed a high rate of macrolide resistance: 45%, among a total of 189 urinary AUC isolates. Genomic analysis identified integrative and conjugative elements (ICEs) as carriers of the macrolide resistance gene , suggesting horizontal gene transfer as a mechanism of resistance. Furthermore, comparison with publicly available genomes of related pathogens revealed high ICE sequence homogeneity, highlighting the potential for cross-species dissemination of resistance determinants. Understanding mechanisms of resistance is crucial for developing effective surveillance strategies and improving antibiotic use. Furthermore, the findings underscore the importance of considering the broader ecological context of resistance dissemination, emphasizing the need for community-level surveillance to combat the spread of antibiotic resistance within the urinary microbiome.
由于对其疾病谱和抗生素耐药性特征了解有限，复杂（AUC）作为一种新兴的尿路病原体被识别后，引发了越来越多的关注。在此，我们调查了尿AUC分离株中大环内酯类耐药性的流行情况，揭示了潜在的遗传机制。表型测试显示，在总共189株尿AUC分离株中，大环内酯类耐药率很高：为45%。基因组分析确定整合和接合元件（ICEs）为大环内酯类耐药基因的载体，表明水平基因转移是耐药机制。此外，与相关病原体的公开可用基因组进行比较，发现ICE序列具有高度同质性，突出了耐药决定因素跨物种传播的可能性。了解耐药机制对于制定有效的监测策略和改善抗生素使用至关重要。此外，研究结果强调了考虑耐药性传播更广泛生态背景的重要性，强调了进行社区层面监测以对抗尿路微生物群中抗生素耐药性传播的必要性。
