DNA亲和柱层析：在从系统性红斑狼疮血清中分离不同抗体群体中的应用。
DNA affinity column chromatography: application in the isolation of distinct antibody populations from SLE sera.
作者信息
Kubota T, Akatsuka T, Kanai Y
出版信息
Clin Exp Immunol. 1985 Nov;62(2):321-8.
An improved method was developed for purification of anti-DNA antibodies by single-stranded DNA (ssDNA), or double-stranded DNA (dsDNA) affinity column chromatography. The effectiveness of this method was examined by measuring the recoveries and purifications of various monoclonal and polyclonal antibodies. Results showed that this method gave good recoveries and purifications of anti-DNA antibodies. Furthermore, by this method, it was possible to isolate antibody populations with distinct specificities from polyclonal antibodies, as shown by enzyme linked immunosorbent assay (ELISA). Therefore, this method should be useful for characterization of polyclonal antibodies in the sera of patients with systemic lupus erythematosus (SLE) as well as of autoimmune lupus mice.
开发了一种改进的方法，通过单链DNA（ssDNA）或双链DNA（dsDNA）亲和柱色谱法纯化抗DNA抗体。通过测量各种单克隆和多克隆抗体的回收率和纯化率来检验该方法的有效性。结果表明，该方法对抗DNA抗体具有良好的回收率和纯化效果。此外，通过该方法，可以从多克隆抗体中分离出具有不同特异性的抗体群体，如酶联免疫吸附测定（ELISA）所示。因此，该方法对于系统性红斑狼疮（SLE）患者血清以及自身免疫性狼疮小鼠血清中多克隆抗体的表征应该是有用的。
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