Rijntjes N V, Van de Putte L B, Van der Pol M, Guelen P J
J Immunol Methods. 1979;30(3):263-8. doi: 10.1016/0022-1759(79)90100-5.
The present report describes a procedure for preparing 4--6 micrometers cryostat sections of undecalcified fresh frozen tissue which contain hard tissue, for immunofluorescence. The apparatus used is a cryomicrotome originally designed for cutting sections for whole body autoradiography. To obtain cryostat sections suitable for tissue immunofluorescence the standard procedure was modified with respect to the hardness and edges of the microtome knife, the temperature of the cryostat and the carboxymethyl cellulose concentration of the embedding material.
本报告描述了一种制备含硬组织的未脱钙新鲜冷冻组织4-6微米低温恒温切片用于免疫荧光的方法。所使用的仪器是一台最初设计用于切割全身放射自显影片切片的冷冻切片机。为了获得适用于组织免疫荧光的低温恒温切片，对切片机刀片的硬度和边缘、低温恒温器的温度以及包埋材料的羧甲基纤维素浓度等标准程序进行了修改。
