Infectious Diseases Unit, Vita-Salute San Raffaele University, 20132 Milan, Italy.
Laboratory of Clinical Microbiology, Virology and Bioemergencies, Ospedale Sacco, 20157 Milan, Italy.
Viruses. 2024 Apr 26;16(5):681. doi: 10.3390/v16050681.
A natural monkeypox virus infection may not induce sufficient neutralizing antibody responses in a subset of healthy individuals. The aim of this study was to evaluate monkeypox virus-neutralizing antibodies six months after infection and to assess the virological factors predictive of a poor immunological response. Antibodies were assessed using a plaque reduction neutralization test at six months from mpox infection; mpox cutaneous, oropharyngeal, and anal swabs, semen, and plasma samples were tested during infection. Overall, 95 people were included in the study; all developed detectable antibodies. People who were positive for the monkeypox virus for more days had higher levels of antibodies when considering all tested samples ( = 0.029) and all swabs ( = 0.005). Mpox cycle threshold values were not predictive of antibody titers. This study found that the overall days of monkeypox virus detection in the body, irrespective of the viral loads, were directly correlated with monkeypox virus neutralizing antibodies at six months after infection.
自然感染猴痘病毒可能不会在一部分健康个体中引起足够的中和抗体反应。本研究旨在评估感染后六个月猴痘病毒中和抗体，并评估预测免疫反应不良的病毒学因素。在感染后六个月，使用蚀斑减少中和试验评估抗体；在感染期间，对猴痘皮肤、口咽和肛门拭子、精液和血浆样本进行检测。总体而言，共有 95 人纳入研究；所有患者均产生可检测到的抗体。考虑到所有检测样本（= 0.029）和所有拭子（= 0.005），猴痘病毒检测阳性天数更多的人抗体水平更高。猴痘病毒循环阈值值不能预测抗体滴度。本研究发现，无论病毒载量如何，感染后六个月，体内猴痘病毒的总检测天数与猴痘病毒中和抗体直接相关。
