开发一种新型血清学检测方法，用于检测接种人群中的猴痘感染。
Development of a novel serological assay for the detection of mpox infection in vaccinated populations.
机构信息
Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, New York, USA.
Department of Biomedical Sciences, The School of Public Heath, The University at Albany, Albany, New York, USA.
出版信息
J Med Virol. 2023 Oct;95(10):e29134. doi: 10.1002/jmv.29134.
Abstract
In 2022 the World Health Organization declared a Public Health Emergency for an outbreak of mpox, the zoonotic Orthopoxvirus (OPV) affecting at least 104 nonendemic locations worldwide. Serologic detection of mpox infection is problematic, however, due to considerable antigenic and serologic cross-reactivity among OPVs and smallpox-vaccinated individuals. In this report, we developed a high-throughput multiplex microsphere immunoassay using a combination of mpox-specific peptides and cross-reactive OPV proteins that results in the specific serologic detection of mpox infection with 93% sensitivity and 98% specificity. The New York State Non-Vaccinia Orthopoxvirus Microsphere Immunoassay is an important tool to detect subclinical mpox infection and understand the extent of mpox spread in the community through retrospective analysis.
摘要
2022 年，世界卫生组织宣布猴痘疫情为国际关注的突发公共卫生事件，这是一种由正痘病毒（OPV）引起的动物源性传染病，影响了全球至少 104 个非流行地区。然而，由于 OPV 之间以及接种过天花疫苗的个体之间存在相当大的抗原和血清交叉反应性，因此对猴痘感染进行血清学检测存在问题。在本报告中，我们开发了一种高通量的微球免疫分析，使用了猴痘特异性肽和交叉反应性 OPV 蛋白的组合，从而实现了对猴痘感染的特异性血清学检测，其灵敏度为 93%，特异性为 98%。纽约州非天花正痘病毒微球免疫分析是一种重要的工具，可以通过回顾性分析来检测亚临床猴痘感染，并了解社区中猴痘传播的程度。
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