Lepe-Zuniga J L, Zigler J S, Zimmerman M L, Gery I
Mol Immunol. 1985 Dec;22(12):1387-92. doi: 10.1016/0161-5890(85)90061-6.
The intracellular (IC) and extracellular (EC) pools of interleukin 1 (IL-1) of human monocyte cultures were found to differ in their molecular size and charge characteristics. EC activity was found by Sephadex G-75 chromatography to consist mainly of a single peak in the 15,000-17,000 mol. wt range. In contrast, IC activity was distributed in four peaks (mol. wts of approx. 15,000, 26,000, 45,000 and greater than 70,000). Treatment of a pool of the IC 26,000, 45,000 and greater than 70,000 mol. wt species with CHAPS, a zwitterionic detergent, yielded a large amount of the 15,000 mol. wt species, thus suggesting that a portion of the larger species consists of aggregates of the 15,000 mol. wt molecule. Both IL-1 pools were found by isoelectrofocusing to be composed of three molecular species with pIs of 5.5, 6.7. However, the proportions of these species differed markedly between the EC and IC pools. The large majority of IC activity (approximately 90%) was found at pI 5.5, while 55-60% of EC activity had a pI of 6.7 and 35-40% had a pI of 5.5. The differences in their biophysical properties support the notion that the IC and EC pools of IL-1 also differ in their functions.
人单核细胞培养物中白细胞介素1（IL-1）的细胞内（IC）池和细胞外（EC）池在分子大小和电荷特性方面存在差异。通过Sephadex G-75色谱法发现，EC活性主要由分子量在15,000 - 17,000范围内的单一峰组成。相比之下，IC活性分布在四个峰中（分子量约为15,000、26,000、45,000和大于70,000）。用两性离子去污剂CHAPS处理IC中分子量为26,000、45,000和大于70,000的组分，产生了大量分子量为15,000的组分，因此表明一部分较大的组分由分子量为15,000的分子聚集体组成。通过等电聚焦发现，两个IL-1池均由三种分子组成，其pI分别为5.5、6.7。然而，这些分子在EC池和IC池中的比例明显不同。在pI 5.5处发现了绝大多数IC活性（约90%），而55 - 60%的EC活性的pI为6.7，35 - 40%的EC活性的pI为5.5。它们生物物理性质的差异支持了IL-1的IC池和EC池在功能上也存在差异的观点。
