Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Thailand Bioresource Research Center (TBRC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang, Pathum Thani 12120, Thailand.
Int J Syst Evol Microbiol. 2024 Jun;74(6). doi: 10.1099/ijsem.0.006410.
During a study on the diversity of culturable actinobacteria from coastal halophytes in Thailand, strain LSe6-5 was isolated from leaves of sea purslane ( L.), and a polyphasic approach was employed to determine its taxonomic position. The 16S rRNA gene sequences analysis indicated that the strain was most closely related to Tu 6233 (99.2 %), YIM M13156 (99.1 %), and PB261 (98.7 %). The genome of strain LSe6-5 was estimated to be 4.33 Mbp in size, with DNA G+C contents of 74.3%. A phylogenomic tree based on whole-genome sequences revealed that strain LSe6-5 formed a clade with DSM 45722, indicating their close relationship. However, the average nucleotide identity (ANI)-blast, ANI-MUMmer, and dDDH values between strain LSe6-5 with DSM 45722 (87.1, 88.9, and 33.0 %) were below the thresholds of 95-96 % ANI and 70 % dDDH for identifying a novel species. Furthermore, strain LSe6-5 showed morphological and chemotaxonomic characteristics of the genus . Cells were motile, rod-shaped, and Gram-stain-positive. Optimal growth of strain LSe6-5 occurred at 28 °C, pH 7.0, and 0-3 % NaCl. The whole-cell hydrolysates contained -diaminopimelic acid as the diagnostic diamino acid, with galactose, glucose, mannose, and ribose as whole-cell sugars. The predominant menaquinones were MK-9(H) and MK-9(H). The polar lipid profile was composed of diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, an unidentified phospholipid, and an unidentified lipid. Major cellular fatty acids were -C, -C, and -C. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, it is supported that strain LSe6-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain LSe6-5 (=TBRC 16417= NBRC 115929).
在对泰国沿海盐生植物可培养放线菌多样性的研究中，从海蓬子（ L. ）叶片中分离到一株 LSe6-5 菌株，并采用多相分类方法确定其分类地位。16S rRNA 基因序列分析表明，该菌株与 Tu 6233（99.2%）、YIM M13156（99.1%）和 PB261（98.7%）最为密切相关。菌株 LSe6-5 的基因组估计大小为 4.33 Mbp，DNA G+C 含量为 74.3%。基于全基因组序列的系统发育树表明，菌株 LSe6-5 与 DSM 45722 形成一个分支，表明它们之间存在密切的关系。然而，菌株 LSe6-5 与 DSM 45722 之间的平均核苷酸同一性（ANI）-blast、ANI-MUMmer 和 dDDH 值（87.1%、88.9%和 33.0%）均低于确定新种的 95-96%ANI 和 70% dDDH 阈值。此外，菌株 LSe6-5 表现出形态学和化学分类学特征属于. 细胞是运动的，杆状，革兰氏阳性。菌株 LSe6-5 的最佳生长条件为 28°C、pH 7.0 和 0-3%NaCl。全细胞水解物含有 -二氨基庚二酸作为诊断二氨基庚二酸，全细胞糖含有半乳糖、葡萄糖、甘露糖和核糖。主要的类异戊二烯醌为 MK-9(H) 和 MK-9(H)。极性脂谱由双磷脂酰甘油、羟磷酰基乙醇胺、磷脂酰肌醇、糖基磷脂酰肌醇、一种未鉴定的磷脂和一种未鉴定的脂组成。主要细胞脂肪酸为 -C、-C 和 -C。基于独特的系统发育位置以及基因型和表型特征的组合，支持菌株 LSe6-5 代表属的一个新种，建议将其命名为. 模式菌株为 LSe6-5 菌株（=TBRC 16417=NBRC 115929）。
