Department of Training Education, Faculty of Sport Sciences, Manisa Celal Bayar University, Manisa, Turkiye.
Department of Biology, Molecular Biology Section, Faculty of Science and Art, Manisa Celal Bayar University, Manisa, Turkiye.
Turk J Med Sci. 2023 Dec 4;54(1):148-156. doi: 10.55730/1300-0144.5775. eCollection 2024.
BACKGROUND/AIM: Although high muscle strength worsens the sense of force, it is unknown whether there is a relationship between this deterioration and the underlying molecular mechanisms. This study examined the relationship between decreased force sense (FS) acuity and strength-related gene expressions.
Maximal voluntary isometric contraction (MVIC) and FS (50% MVIC) tests were performed on the knee joints of twenty-two subjects. The expression analyses were evaluated by qRT-PCR in blood samples taken before, after MVIC, after 50% MVIC, and 15 min after the test.
MVIC and FS error values were significantly correlated with each other (r = .659, p = .001). The qRT-PCR analyses demonstrated that the expressed mRNAs of the interleukin 6 (IL-6), alpha-actinin 3 (ACTN3), angiotensin-converting enzyme (ACE), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor receptor (CNTFR) genes dramatically increased until 50% MVIC and subsequently decreased 15 min after the exercise (p < .05). The muscle-specific creatine kinase (CKMM), myosin light chain kinase (MLCK), and G-protein β3 subunit (GNB3) genes reached their peak expression levels 30 min after MVIC (p < .05). ACE and ACTN3 gene expression increased significantly in parallel with the increased FS error (p < .05). These gene expression fluctuations observed at 50% MVIC and after the rest could be related to changes in cellular metabolism leading to fatigue.
The time points of gene expression levels during exercise need to be considered. The force acuity of those whose maximal force develops too much may deteriorate.
背景/目的：虽然高肌肉力量会降低力感，但目前尚不清楚这种恶化是否与潜在的分子机制有关。本研究探讨了力感敏锐度降低与与力量相关的基因表达之间的关系。
对 22 名受试者的膝关节进行最大自主等长收缩（MVIC）和力感（50%MVIC）测试。在进行 MVIC 之前、之后、50%MVIC 之后和测试后 15 分钟，通过 qRT-PCR 评估血液样本中的表达分析。
MVIC 和 FS 误差值彼此显著相关（r =.659，p =.001）。qRT-PCR 分析表明，白细胞介素 6（IL-6）、α-辅肌动蛋白 3（ACTN3）、血管紧张素转换酶（ACE）、脑源性神经营养因子（BDNF）和睫状神经营养因子受体（CNTFR）基因的表达 mRNA 在达到 50%MVIC 之前急剧增加，随后在运动后 15 分钟下降（p <.05）。肌肉特异性肌酸激酶（CKMM）、肌球蛋白轻链激酶（MLCK）和 G 蛋白β3 亚基（GNB3）基因在 MVIC 后 30 分钟达到表达峰值（p <.05）。ACE 和 ACTN3 基因表达与 FS 误差的增加呈显著正相关（p <.05）。这些在 50%MVIC 时观察到的基因表达波动以及休息后的波动可能与导致疲劳的细胞代谢变化有关。
需要考虑运动期间基因表达水平的时间点。那些最大力量发展过多的人的力感敏锐度可能会恶化。
