Sun I L, Crane F L, Grebing C, Löw H
Exp Cell Res. 1985 Feb;156(2):528-36. doi: 10.1016/0014-4827(85)90559-2.
The impermeable electron acceptor ferricyanide stimulates the growth of HeLa cells in the absence of serum and increases cell replication with limiting amounts of serum (0.75%). Maximum growth stimulation occurs at low ferricyanide concentration from 0.01 to 0.1 mM. Higher ferricyanide concentrations inhibit growth on serum. Addition of insulin enhances the stimulating effect of ferricyanide. Increase in the transplasmalemma electron transport activity in the presence of insulin is also observed by measuring the rate of ferricyanide reduction by cells. There is a close correlation between insulin stimulation of ferricyanide reduction and insulin induction of cell proliferation and attachment. In addition to ferricyanide, the growth response is observed with other impermeable oxidants, such as indigotetrasulfonate and hexaamine ruthenium III, which are reduced by the transplasma membrane electron transport system. Inactive oxidants such as cytochrome c do not stimulate cell growth. Ferrocyanide does not stimulate growth. We propose that electron flow through the transplasma membrane electron transport system stimulates growth and that insulin acts to increase that flow.
不可渗透的电子受体铁氰化物在无血清条件下刺激HeLa细胞生长，并在血清含量有限（0.75%）时增加细胞复制。在0.01至0.1 mM的低铁氰化物浓度下出现最大生长刺激。更高的铁氰化物浓度会抑制血清存在时的生长。添加胰岛素可增强铁氰化物的刺激作用。通过测量细胞还原铁氰化物的速率，还观察到在胰岛素存在下跨质膜电子转运活性增加。胰岛素刺激铁氰化物还原与胰岛素诱导细胞增殖和附着之间存在密切相关性。除铁氰化物外，其他不可渗透的氧化剂如靛蓝四磺酸盐和六胺钌（III）也观察到生长反应，它们可被跨质膜电子转运系统还原。无活性的氧化剂如细胞色素c不会刺激细胞生长。亚铁氰化物不会刺激生长。我们提出，通过跨质膜电子转运系统的电子流刺激生长，而胰岛素的作用是增加这种电子流。
