Zhao Fan, Yan Linlin, Wang Pengfei, Zhang Ke, Hu Shoukui
Department of Clinical Laboratory, Peking University Shougang Hospital, Beijing, 100144, China.
Department of Clinical Laboratory, Liangxiang Hospital of Fangshan District, Beijing, 102400, China.
Heliyon. 2024 May 18;10(10):e31472. doi: 10.1016/j.heliyon.2024.e31472. eCollection 2024 May 30.
() plays a major role in causing and advancing gastrointestinal illnesses. Our aim is to analyze the unique makeup and functional changes in the gastric microbiota of patients with chronic non-atrophic gastritis (CNAG), regardless of the presence of , and to determine the potential signaling pathways.
We performed metagenomic sequencing on gastric mucosa samples collected from 17 individuals with non-atrophic gastritis, comprising 6 cases were infected with (-infected case group) and 11 cases without (control group). The species composition was evaluated with DIAMOND software, and functional enrichment was assessed utilizing the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. We analyzed antibiotic resistance patterns using the Comprehensive Antibiotic Resistance Database as a reference (CARD).
The presence of colonization in CNAG patients was associated with increased diversity in the gastric microbiota. The Phylum Firmicutes was found to be less prevalent, while the Phylum Proteobacteria showed an increase. Functionally, pathways associated with metabolic pathways, including vitamins, auxiliaries, amino acid residue, carbon hydrate, and metabolic energy pathways, were enriched in CNAG patients with infection. Additionally, antibiotic resistance genes correlated with antibiotic efflux pump were enriched.
From a holistic genomic perspective, our findings offer fresh perspectives into the gastric microbiome among CNAG patients carrying , which is valuable for future research on CNAG.
（）在胃肠道疾病的发生和发展中起主要作用。我们的目的是分析慢性非萎缩性胃炎（CNAG）患者胃微生物群的独特组成和功能变化，无论是否存在（），并确定潜在的信号通路。
我们对从17例非萎缩性胃炎患者收集的胃黏膜样本进行了宏基因组测序，其中包括6例感染（）的病例（感染病例组）和11例未感染的病例（对照组）。使用DIAMOND软件评估物种组成，并利用京都基因与基因组百科全书（KEGG）数据库评估功能富集情况。我们以综合抗生素抗性数据库（CARD）为参考分析抗生素抗性模式。
CNAG患者中（）定植的存在与胃微生物群多样性增加有关。发现厚壁菌门的流行率较低，而变形菌门则有所增加。在功能上，与代谢途径相关的通路，包括维生素、辅助因子、氨基酸残基、碳水化合物和代谢能量途径，在感染（）的CNAG患者中富集。此外，与抗生素外排泵相关的抗生素抗性基因也得到了富集。
从整体基因组学角度来看，我们的研究结果为携带（）的CNAG患者的胃微生物组提供了新的视角，这对未来CNAG的研究具有重要价值。
