Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu 610065, PR China; National Engineering Research Center of Clean Technology in Leather Industry, Sichuan University, Chengdu 610065, PR China.
Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu 610065, PR China; National Engineering Research Center of Clean Technology in Leather Industry, Sichuan University, Chengdu 610065, PR China.
Carbohydr Polym. 2024 Nov 1;343:122409. doi: 10.1016/j.carbpol.2024.122409. Epub 2024 Jun 19.
The study focuses on developing a bioactive shape memory sponge to address the urgent demand for short-term rapid hemostasis and long-term wound healing in noncompressible hemorrhage cases. A composite sponge was created by spontaneously generating pores and double cross-linking under mild conditions using biomimetic collagen fibril (BCF) and oxidized alginate (OA) as natural backbone, combined with an inert calcium source (Ca) from CaCO-GDL slow gelation mechanism. The optimized BCF/OACa (5/5) sponge efficiently absorbed blood after compression and recovered to its original state within 11.2 ± 1.3 s, achieving physical hemostatic mechanism. The composite sponge accelerated physiological coagulation by promoting platelet adhesion and activation through BCF, as well as enhancing endogenous and exogenous hemostatic pathways by Ca. Compared to commercial PVA expanding hemostatic sponge, the composite sponge reduced bleeding volume and shortened hemostasis time in rat liver injury pick and perforation wound models. Additionally, it stimulated fibroblast migration and differentiation, thus promoting wound healing. It is biodegradable with low inflammatory response and promotes granulation tissue regeneration. In conclusion, this biocomposite sponge provides multiple hemostatic pathways and biochemical support for wound healing, is biologically safe and easy to fabricate, process and use, with significant potential for clinical translation and application.
该研究旨在开发一种具有生物活性的形状记忆海绵，以满足非压迫性出血情况下短期快速止血和长期伤口愈合的迫切需求。通过仿生胶原蛋白纤维（BCF）和氧化海藻酸钠（OA）作为天然骨架，在温和条件下自发产生孔和双重交联，并结合 CaCO3-GDL 缓慢胶凝机制中的惰性钙源（Ca），制备了一种复合海绵。优化后的 BCF/OA-Ca（5/5）海绵在压缩后能有效吸收血液，并在 11.2±1.3s 内恢复到原来的状态，实现了物理止血机制。该复合海绵通过 BCF 促进血小板黏附和激活，增强内源性和外源性止血途径，从而加速生理凝血。与商用 PVA 膨胀性止血海绵相比，复合海绵在大鼠肝损伤穿刺和穿孔伤口模型中减少了出血量并缩短了止血时间。此外，它还刺激成纤维细胞迁移和分化，从而促进伤口愈合。它具有生物降解性、低炎症反应，并能促进肉芽组织再生。综上所述，这种生物复合材料海绵为伤口愈合提供了多种止血途径和生化支持，具有良好的生物安全性，易于制造、加工和使用，具有重要的临床转化和应用潜力。
