Centre for Bio Separation Technology (CBST), Technology Tower, Vellore Institute of Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Protein J. 2024 Aug;43(4):656-674. doi: 10.1007/s10930-024-10205-0. Epub 2024 Jun 2.
Modern medicine has increased the human lifespan. However, with an increase in average lifespan risk of amyloidosis increases. Amyloidosis is a condition characterized by protein misfolding and aggregation. Early detection of amyloidosis is crucial, yet conventional diagnostic methods are costly and lack precision, necessitating innovative tools. This review explores recent advancements in diverse amyloid detection methodologies, highlighting the need for interdisciplinary research to develop a miniaturized electrochemical biosensor leveraging nanotechnology. However, the diagnostics industry faces obstacles such as skilled labor shortages, standardized selection processes, and concurrent multi-analyte identification challenges. Research efforts are focused on integrating electrochemical techniques into clinical applications and diagnostics, with the successful transition of miniaturized technologies from development to testing posing a significant hurdle. Label-free transduction techniques like voltammetry and electrochemical impedance spectroscopy (EIS) have gained traction due to their rapid, cost-effective, and user-friendly nature.
现代医学延长了人类的寿命。然而，随着平均寿命的增加，淀粉样变性的风险也在增加。淀粉样变性是一种以蛋白质错误折叠和聚集为特征的疾病。淀粉样变性的早期检测至关重要，但传统的诊断方法既昂贵又缺乏准确性，因此需要创新的工具。本综述探讨了不同的淀粉样检测方法的最新进展，强调需要开展跨学科研究，利用纳米技术开发一种小型化的电化学生物传感器。然而，诊断行业面临着一些障碍，如熟练劳动力短缺、标准化的选择过程以及同时进行多分析物识别的挑战。研究工作集中在将电化学技术整合到临床应用和诊断中，成功地将小型化技术从开发阶段过渡到测试阶段是一个重大的挑战。基于伏安法和电化学阻抗谱（EIS）等无标记转导技术由于其快速、经济高效和用户友好的特点而受到关注。
