Cryz S J, Fürer E, Germanier R
J Infect Dis. 1985 Apr;151(4):665-71. doi: 10.1093/infdis/151.4.665.
The safety and immunogenicity of two Klebsiella pneumoniae K1 capsular polysaccharide (CPS) vaccines were evaluated in humans. Trace quantities of lipopolysaccharide present in vaccine preparations were detoxified by treatment of K1 CPS in a 95% ethanol-0.1 N NaOH solution. This procedure greatly reduced the pyrogenicity of K1 CPS but did not markedly alter its antigenicity, molecular size, or immunogenicity for animals. Volunteers received either 25 or 50 micrograms of untreated or NaOH-treated K1 CPS vaccine subcutaneously. Systemic reactions on primary vaccination were infrequent with both vaccine preparations. However, the frequency and severity of local reactions were substantially reduced after immunization with NaOH-treated vaccine as compared with untreated K1 CPS. All vaccinees responded with a fourfold or greater rise in IgG and IgM titers. IgG antibody to K1 CPS isolated from immune sera was highly effective in preventing fatal experimental burn wound sepsis due to K. pneumoniae K1 in mice.
在人体中评估了两种肺炎克雷伯菌K1荚膜多糖（CPS）疫苗的安全性和免疫原性。通过在95%乙醇-0.1N氢氧化钠溶液中处理K1 CPS，对疫苗制剂中存在的微量脂多糖进行解毒。该程序大大降低了K1 CPS的致热原性，但未明显改变其抗原性、分子大小或对动物的免疫原性。志愿者皮下接种25或50微克未处理或经氢氧化钠处理的K1 CPS疫苗。两种疫苗制剂初次接种时的全身反应都不常见。然而，与未处理的K1 CPS相比，用经氢氧化钠处理的疫苗免疫后，局部反应的频率和严重程度大幅降低。所有接种疫苗者的IgG和IgM滴度均有四倍或更高的升高。从免疫血清中分离出的针对K1 CPS的IgG抗体在预防小鼠因肺炎克雷伯菌K1引起的致命实验性烧伤创面败血症方面非常有效。
