Cryz S J, Mortimer P, Cross A S, Fürer E, Germanier R
Vaccine. 1986 Mar;4(1):15-20. doi: 10.1016/0264-410x(86)90092-7.
A polyvalent Klebsiella vaccine composed of six serotypes of capsular polysaccharides (K2, K3, K10, K21, K30, and K55) was developed and its safety and immunogenicity evaluated in humans. Highly purified capsular antigens were treated in 0.1 N NaOH in 95% ethanol to detoxify trace amounts of contaminating lipopolysaccharide (LPS). The vaccine was nontoxic and nonpyrogenic for animals. A total of 40 individuals received either 25 or 50 micrograms of each represented antigen subcutaneously. Reactions to vaccination, where noted, were transient and mild in nature. An immunizing dose of 50 micrograms of each antigen (300 micrograms total) elicited a fourfold or greater immunoglobulin G (IgG) response to all vaccine antigens in greater than 80% of vaccinees. Generally, the serospecificity of the antibody response was limited to those capsular antigens included in the vaccine. IgG isolated from the serum of vaccinees was found to be highly protective against fatal experimental Klebsiella K2 burn wound sepsis indicating that the functional antibody is elicited following vaccination.
一种由六种荚膜多糖血清型（K2、K3、K10、K21、K30和K55）组成的多价克雷伯菌疫苗被研发出来，并在人体中评估了其安全性和免疫原性。高度纯化的荚膜抗原在95%乙醇中的0.1N氢氧化钠中处理，以去除痕量污染的脂多糖（LPS）。该疫苗对动物无毒且无致热原性。共有40名个体皮下注射了25微克或50微克每种代表性抗原。记录到的疫苗接种反应短暂且性质轻微。每种抗原50微克（总计300微克）的免疫剂量在超过80%的接种者中引发了对所有疫苗抗原四倍或更高的免疫球蛋白G（IgG）反应。一般来说，抗体反应的血清特异性仅限于疫苗中包含的那些荚膜抗原。从接种者血清中分离出的IgG被发现对致命的实验性克雷伯菌K2烧伤创面败血症具有高度保护作用，这表明接种疫苗后可引发功能性抗体。
