Schooley J C, Kullgren B, Fletcher B L
Int J Cell Cloning. 1985 Jan;3(1):2-9. doi: 10.1002/stem.5530030103.
Successful culture of hematopoietic cells depends upon the growth of stromal cells. The growth of bone marrow stromal cells is best achieved when hydrocortisone is added to the culture medium. However, the hydrocortisone requirement is eliminated if the cultures are grown in an atmosphere of 5% CO2, 5% O2, and 90% N2 rather than 5% CO2 in air, which is more common.
造血细胞的成功培养取决于基质细胞的生长。当向培养基中添加氢化可的松时，骨髓基质细胞的生长效果最佳。然而，如果培养是在5%二氧化碳、5%氧气和90%氮气的气氛中进行，而不是在空气中5%二氧化碳（这更常见）的环境中进行，那么氢化可的松的需求就可以消除。
