School of Food and Biological Engineering, Jiangsu University, Zhenjiang, PR China.
School of Agricultural Engineering, Jiangsu University, Zhenjiang, PR China.
Int J Syst Evol Microbiol. 2024 Jun;74(6). doi: 10.1099/ijsem.0.006402.
The genera and are phylogenetically related genera within the family . Species of these genera were described using 16S rRNA gene-based phylogeny and phenotypic characteristics. However, the 16S rRNA gene identity and phylogeny reveal the controversy of the taxonomic status of these two genera. In this work, we examined the taxonomic positions of members of both genera using 16S rRNA gene phylogeny, phylogenomic analysis and further validated using overall genome-related indexes, including digital DNA-DNA hybridization, average nucleotide identity, average amino acid identity and percentage of conserved proteins. Based on phylogenetic and phylogenomic results, the current four species of the two genera clustered tightly into one clade with high bootstrap values, suggesting that the genus should be merged with . In addition, a novel species isolated from a soda soil sample collected from Anda City, PR China, and designated as HJB301 was also described. Phenotypic, chemotaxonomic, genomic and phylogenetic properties suggested that strain HJB301 (=CCTCC AB 2021113=KCTC 82977) represents a novel species of the genus , for which the name sp. nov. is proposed.
属和属是科内系统发育上相关的属。这些属的物种是根据 16S rRNA 基因系统发育和表型特征描述的。然而，16S rRNA 基因的同一性和系统发育揭示了这两个属的分类地位存在争议。在这项工作中，我们使用 16S rRNA 基因系统发育、基因组分析进一步验证了这两个属成员的分类地位，包括全基因组相关指标，包括数字 DNA-DNA 杂交、平均核苷酸同一性、平均氨基酸同一性和保守蛋白百分比。基于系统发育和基因组分析的结果，这两个属的目前四个种紧密聚类成一个具有高支值的分支，表明属应该与合并。此外，还从中国河南省安阳市采集的苏打土壤样本中分离到一株新的菌株 HJB301，并命名为 HJB301(=CCTCC AB 2021113=KCTC 82977)。表型、化学分类、基因组和系统发育特性表明，HJB301 菌株代表了属的一个新种，建议将其命名为 sp. nov.。
