Yang Renhao, Xu Yidong, Li Renxuan, Zhang Yin, Xu Yang, Yang Liuquan, Cui Wenguo, Wang Lei
Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Department of Orthopedics, Sports Medicine Center, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
School of Mechanical Engineering, University of Leeds, Leeds, LS2 9JT, U.K.
Nano Lett. 2024 Jun 4;24(24):7381-8. doi: 10.1021/acs.nanolett.4c01540.
Inspired by the imbalance between extrinsic and intrinsic tendon healing, this study fabricated a new biofilter scaffold with a hierarchical structure based on a melt electrowriting technique. The outer multilayered fibrous structure with connected porous characteristics provides a novel passageway for vascularization and isolates the penetration of scar fibers, which can be referred to as a biofilter process. In vitro experiments found that the porous architecture in the outer layer can effectively prevent cell infiltration, whereas the aligned fibers in the inner layer can promote cell recruitment and growth, as well as the expression of tendon-associated proteins in a simulated friction condition. It was shown in vivo that the biofilter process could promote tendon healing and reduce scar invasion. Herein, this novel strategy indicates great potential to design new biomaterials for balancing extrinsic and intrinsic healing and realizing scarless tendon healing.
受外在和内在肌腱愈合之间不平衡的启发，本研究基于熔体静电纺丝技术制造了一种具有分级结构的新型生物过滤支架。具有连通多孔特征的外层多层纤维结构为血管化提供了一条新通道，并隔离了瘢痕纤维的侵入，这一过程可称为生物过滤过程。体外实验发现，外层的多孔结构可有效防止细胞浸润，而内层的排列纤维可促进细胞募集和生长，以及在模拟摩擦条件下肌腱相关蛋白的表达。体内实验表明，生物过滤过程可促进肌腱愈合并减少瘢痕侵入。在此，这种新策略显示出设计新型生物材料以平衡外在和内在愈合并实现无瘢痕肌腱愈合的巨大潜力。
