Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Chem Commun (Camb). 2024 Jun 20;60(51):6508-6511. doi: 10.1039/d3cc04919a.
Cyclotides and lanthipeptides are cyclic peptide natural products with promising bioengineering potential. No peptides have been isolated that contain both structural motifs defining these two families, an N-to-C cyclised backbone and lanthionine linkages. We combined their biosynthetic machineries to produce hybrid structures that possess improved activity or stability, demonstrate how the AEP-1 plant cyclase can be utilised to complete the maturation of the sactipeptide subtilosin A, and present head-to-tail cyclisation of the glycocin sublancin. These studies show the plasticity of AEP-1 and its utilisation alongside other post-translational modifications.
环肽和硫肽是具有广阔生物工程应用前景的环状肽天然产物。尚未分离到同时包含这两种家族结构特征的肽，即 N 到 C 环化的骨架和硫醚键。我们将它们的生物合成机制结合起来，产生具有改进的活性或稳定性的杂合结构，展示了 AEP-1 植物环化酶如何用于完成 sactipeptide 枯草菌素 A 的成熟，并呈现甘糖菌素 sublancin 的头对头环化。这些研究表明了 AEP-1 的可塑性及其与其他翻译后修饰的结合使用。
