Krikos A, Conley M P, Boyd A, Berg H C, Simon M I
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1326-30. doi: 10.1073/pnas.82.5.1326.
The tar and tsr genes of Escherichia coli encode homologous transducer proteins that mediate distinct chemotactic responses. We report here the construction of two tasr chimeric genes in which the 5' coding region of the tar gene is fused to the 3' coding region of the tsr gene at either of two conserved restriction sites. Both chimeric genes code for chemotactically functional proteins. Results of analyses of behavior and methylation in cells carrying the chimeric genes support existing models for the disposition of transducer domains across the cell membrane and reveal that the receptors for internal pH map in a specific region of the COOH-terminal (cytoplasmic) domain.
大肠杆菌的tar和tsr基因编码同源转导蛋白，介导不同的趋化反应。我们在此报告构建了两个tasr嵌合基因，其中tar基因的5'编码区在两个保守的限制性位点之一处与tsr基因的3'编码区融合。两个嵌合基因都编码具有趋化功能的蛋白质。对携带嵌合基因的细胞行为和甲基化分析的结果支持了关于跨细胞膜转导结构域分布的现有模型，并揭示了细胞内pH受体定位在COOH末端（细胞质）结构域的特定区域。
