Veterinery Public Health and Epidemology Division, School of Veterinary Medicine and Biomedical Sciences (SVMBS), IPB University, Bogor 16680, Indonesia.
Animal Health Division, Bogor Agricultural Development Polytechnic, Bogor 16730, Indonesia.
J Vet Sci. 2024 May;25(3):e44. doi: 10.4142/jvs.24031.
The emergence and rapid increase in the incidence of multidrug-resistant (MDR) bacteria in pig farms has become a serious concern and reduced the choice of effective antibiotics.
This study analyzed the phylogenetics and diversity of antibiotic resistance genes (ARGs) and molecularly identified the source of ARGs in antibiotic-resistant isolated from pig farms in Banten Province, Indonesia.
Forty-four antibiotic-resistant isolates from fecal samples from 44 pig farms in Banten Province, Indonesia, were used as samples. The samples were categorized into 14 clusters. Sequencing was performed using the Oxford Nanopore Technologies MinION platform, with barcoding before sequencing with Nanopore Rapid sequencing gDNA-barcoding (SQK-RBK110.96) according to manufacturing procedures. ARG detection was conducted using ResFinder, and the plasmid replicon was determined using PlasmidFinder.
Three phylogenetic leaves of were identified in the pig farming cluster in Banten Province. The isolates exhibited potential resistance to nine classes of antibiotics. Fifty-one ARGs were identified across all isolates, with each cluster carrying a minimum of 10 ARGs. The and genes were present in all isolates. ARGs in the pig farming cluster originated mainly from plasmids, accounting for an average of 89.4%.
The elevated potential for MDR events, coupled with the dominance of ARGs originating from plasmids, increases the risk of ARG spread among bacterial populations in animals, humans, and the environment.
养殖场中多药耐药（MDR）细菌的出现和迅速增加已成为一个严重的问题，降低了有效抗生素的选择。
本研究分析了印度尼西亚万丹省养殖场中分离的耐药 的抗生素耐药基因（ARGs）的系统发育和多样性，并通过分子鉴定确定了 ARGs 的来源。
从印度尼西亚万丹省 44 个养猪场的粪便样本中分离出 44 株抗生素耐药 作为样本。这些样本被分为 14 个聚类。使用 Oxford Nanopore Technologies MinION 平台进行测序，在测序前使用 Nanopore Rapid sequencing gDNA-barcoding（SQK-RBK110.96）进行条形码标记，根据制造程序进行。使用 ResFinder 进行 ARG 检测，使用 PlasmidFinder 确定质粒复制子。
在万丹省养猪场聚类中鉴定出 3 个 的系统发育叶。 分离株对九类抗生素表现出潜在的耐药性。在所有分离株中鉴定出 51 个 ARGs，每个聚类至少携带 10 个 ARGs。 和 基因存在于所有分离株中。养猪场聚类中的 ARGs 主要来源于质粒，平均占 89.4%。
MDR 事件的风险增加，加上主要来源于质粒的 ARGs 的存在，增加了动物、人类和环境中细菌种群中 ARG 传播的风险。
