Whole genome sequencing analysis on antibiotic-resistant isolated from pig farms in Banten Province, Indonesia.

IMPORTANCE

The emergence and rapid increase in the incidence of multidrug-resistant (MDR) bacteria in pig farms has become a serious concern and reduced the choice of effective antibiotics.

OBJECTIVE

This study analyzed the phylogenetics and diversity of antibiotic resistance genes (ARGs) and molecularly identified the source of ARGs in antibiotic-resistant isolated from pig farms in Banten Province, Indonesia.

METHODS

Forty-four antibiotic-resistant isolates from fecal samples from 44 pig farms in Banten Province, Indonesia, were used as samples. The samples were categorized into 14 clusters. Sequencing was performed using the Oxford Nanopore Technologies MinION platform, with barcoding before sequencing with Nanopore Rapid sequencing gDNA-barcoding (SQK-RBK110.96) according to manufacturing procedures. ARG detection was conducted using ResFinder, and the plasmid replicon was determined using PlasmidFinder.

RESULTS

Three phylogenetic leaves of were identified in the pig farming cluster in Banten Province. The isolates exhibited potential resistance to nine classes of antibiotics. Fifty-one ARGs were identified across all isolates, with each cluster carrying a minimum of 10 ARGs. The and genes were present in all isolates. ARGs in the pig farming cluster originated mainly from plasmids, accounting for an average of 89.4%.

CONCLUSIONS AND RELEVANCE

The elevated potential for MDR events, coupled with the dominance of ARGs originating from plasmids, increases the risk of ARG spread among bacterial populations in animals, humans, and the environment.