Shaanxi Key Laboratory of Bio-Resources, School of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong, 723000, Shaanxi, China.
Sci Rep. 2022 Mar 9;12(1):4164. doi: 10.1038/s41598-022-08172-6.
The genus Eristalinus is widely distributed globally. Four complete mitochondrial genomes (i.e., mitogenomes) of Eristalinus were sequenced and analyzed in this study: Eristalinus viridis (Coquillett, 1898), E. quinquestriatus (Fabricius, 1781), E. tarsalis (Macquart, 1855), and E. sp. Within these four sequenced mitogenomes, most protein-coding genes (ND2, CO1, COX2, COX3, ND3, ND5, ND4, ND4L, ND6, and Cytb) began with a typical ATN (T/C/G/A) start codon and ended with a stop codon TAA or incomplete T, whereas ND1 began with the start codon TTG. ND3 ended with TAG. The secondary tRNA structure was that of a typical cloverleaf, and only the tRNA-Ser1 lacked a DHU arm. Three and five domains appeared in the 12S and 16S rRNA secondary structures, respectively. The phylogenetic relationships among the four Eristalinus species combined with the published mitogenomes of Syrphidae were reconstructed using the maximum likelihood and Bayesian inference methods, which support the monophyly of the subfamily Syrphinae but do not support that of the subfamily Eristalinae. Of note, Eristalini and Syrphini are monophyletic groups. The mitogenomes of E. viridis, E. quinquestriatus, E. sp., and E. tarsalis are useful for determining the phylogenetic relationships and evolution of Syrphidae.
绿盲蝽属广泛分布于全球。本研究对 4 种绿盲蝽属完整的线粒体基因组（即线粒体基因组）进行了测序和分析：Eristalinus viridis（Coquillett，1898）、E. quinquestriatus（Fabricius，1781）、E. tarsalis（Macquart，1855）和 E. sp. 在这 4 个测序的线粒体基因组中，大多数蛋白质编码基因（ND2、CO1、COX2、COX3、ND3、ND5、ND4、ND4L、ND6 和 Cytb）以典型的 ATN（T/C/G/A）起始密码子开始，以 TAA 或不完整的 T 终止密码子结束，而 ND1 以起始密码子 TTG 开始。ND3 以 TAG 结尾。二级 tRNA 结构为典型的三叶形，只有 tRNA-Ser1 缺乏 DHU 臂。12S 和 16S rRNA 的二级结构分别出现 3 个和 5 个结构域。使用最大似然法和贝叶斯推断法重建了这 4 种绿盲蝽属与已发表的食蚜蝇科线粒体基因组的系统发育关系，支持了食蚜蝇亚科的单系性，但不支持盲蝽亚科的单系性。值得注意的是，Eristalini 和 Syrphini 是单系群。E. viridis、E. quinquestriatus、E. sp. 和 E. tarsalis 的线粒体基因组有助于确定食蚜蝇科的系统发育关系和进化。
