DeFeo-Jones D, Tatchell K, Robinson L C, Sigal I S, Vass W C, Lowy D R, Scolnick E M
Science. 1985 Apr 12;228(4696):179-84. doi: 10.1126/science.3883495.
Activated versions of ras genes have been found in various types of malignant tumors. The normal versions of these genes are found in organisms as diverse as mammals and yeasts. Yeast cells that lack their functional ras genes, RASSC-1 and RASSC-2, are ordinarily nonviable. They have now been shown to remain viable if they carry a mammalian rasH gene. In addition, yeast-mammalian hybrid genes and a deletion mutant yeast RASSC-1 gene were shown to induce morphologic transformation of mouse NIH 3T3 cells when the genes had a point mutation analogous to one that increases the transforming activity of mammalian ras genes. The results establish the functional relevance of the yeast system to the genetics and biochemistry of cellular transformation induced by mammalian ras genes.
在各类恶性肿瘤中均发现了激活状态的ras基因。这些基因的正常版本存在于诸如哺乳动物和酵母等多种生物体内。缺乏功能性ras基因RASSC - 1和RASSC - 2的酵母细胞通常无法存活。现已证明，如果这些酵母细胞携带哺乳动物rasH基因，它们就能存活。此外，当酵母 - 哺乳动物杂交基因和缺失突变酵母RASSC - 1基因发生类似于增加哺乳动物ras基因转化活性的点突变时，可诱导小鼠NIH 3T3细胞发生形态转化。这些结果证实了酵母系统与哺乳动物ras基因诱导的细胞转化的遗传学和生物化学之间的功能相关性。
