Harlozinska A, Kula J, Stepinska B, Jelen M, Richter R, Slesak B
Am J Clin Pathol. 1985 Mar;83(3):301-7. doi: 10.1093/ajcp/83.3.301.
The distribution of cervical carcinoma antigens (AgCaCx), CEA, and NCA in different pathologic states of the uterine cervix was studied in cytologic smears by an immunofluorescence method (IF) using specific immune sera against perchloric acid (PCA) extract of cervical squamous cell carcinoma, anti-CEA, and anti-NCA. After excluding cross-reactivity with CEA and NCA, the presence of AgCaCx was demonstrated in the majority of cervical carcinomas, severe dysplasias, and only in one-fourth of squamous metaplasias, especially when accompanied by mild or moderate dysplasias. The intensity and percentage of IF-positive cells varied from case to case. The preparations of uterine cervix without pathologic changes usually were negative. Similar results were obtained with anti-CEA serum. NCA was present in the majority of smears independent of histologic diagnosis. The most intense fluorescence was observed in upper layers of the epithelium. NCA could be a differentiation antigen of stratified squamous cell epithelium.
采用针对宫颈鳞状细胞癌高氯酸（PCA）提取物的特异性免疫血清、抗癌胚抗原（CEA）和抗非癌胚抗原（NCA），通过免疫荧光法（IF）研究了子宫颈不同病理状态下宫颈癌抗原（AgCaCx）、CEA和NCA在细胞学涂片中的分布。在排除与CEA和NCA的交叉反应后，在大多数宫颈癌、重度发育异常中均证实存在AgCaCx，而仅在四分之一的鳞状化生中存在，尤其是伴有轻度或中度发育异常时。IF阳性细胞的强度和百分比因病例而异。无病理改变的子宫颈涂片通常为阴性。抗CEA血清也得到了类似结果。大多数涂片均存在NCA，与组织学诊断无关。在上皮上层观察到最强的荧光。NCA可能是复层鳞状上皮的一种分化抗原。
