Dezfulian M, Bartlett J G
Diagn Microbiol Infect Dis. 1985 Mar;3(2):105-12. doi: 10.1016/0732-8893(85)90018-5.
A low molecular weight substance that induced seizures and death in mice was present in the stool of a child with infant botulism. This interfered with mouse bioassay used for detecting Clostridium botulinum toxin. After removal of the interfering substance by a prolonged dialysis with buffered saline, in vivo neutralization of botulinal toxin was readily achieved. Alternatively, the botulinal toxin was demonstrated in stool of the infant by an enzyme-linked immunosorbent assay (ELISA), using antibodies produced in immunologically tolerant rabbits.
一名患有婴儿肉毒中毒的儿童粪便中存在一种能在小鼠中诱发癫痫发作和死亡的低分子量物质。这干扰了用于检测肉毒梭菌毒素的小鼠生物测定法。在用缓冲盐水进行长时间透析去除干扰物质后，肉毒毒素的体内中和很容易实现。或者，通过酶联免疫吸附测定法（ELISA），使用在免疫耐受兔中产生的抗体，在婴儿粪便中检测到了肉毒毒素。
