无义突变对真核生物信使核糖核酸稳定性的干扰。
Interference of nonsense mutations with eukaryotic messenger RNA stability.
作者信息
Losson R, Lacroute F
出版信息
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5134-7. doi: 10.1073/pnas.76.10.5134.
Abstract
The fine structure map of the yeast URA 3 gene was established by meiotic recombination, and amber nonsense mutations were located at different points on the map. The effect of the length of the labeling time on the specific radioactivity of ura 3 messenger RNA and on its repartition between poly(A)-RNA and RNA not containing poly(A) has been followed in nonsense mutants. Nonsense mutations reduce the messenger level without lowering its instantaneous rate of synthesis. The strength of the reduction depends on the position of the nonsense codon within the locus and concerns essentially the accumulation of polyadenylylated ura 3 mRNA.
摘要
通过减数分裂重组建立了酵母URA 3基因的精细结构图谱，琥珀色无义突变位于该图谱的不同位点。在无义突变体中，研究了标记时间长度对ura 3信使RNA的比放射性及其在聚腺苷酸RNA和不含聚腺苷酸的RNA之间分配的影响。无义突变降低了信使水平，但不降低其瞬时合成速率。降低的强度取决于无义密码子在基因座内的位置，并且主要涉及聚腺苷酸化的ura 3 mRNA的积累。
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