Ludwig J R, Oliver S G, McLaughlin C S
Mol Gen Genet. 1977 Dec 30;158(2):117-22. doi: 10.1007/BF00268303.
A study has been made of the effects of a casamino acids shift-up on a prototrophic strain of yeast growing under conditions of ammonium repression. The shift-up produced an increase in growth rate some 120 min after the addition of amino acids to the medium. This growth rate increase was slightly preceded by an increase in the rate of accumulation of DNA. In contrast, the rate of accumulation of protein increased immediately and that of RNA 15-20 min after the shift. RNA was initially accumulated at a rate greater than that required to sustain the new steady state. This was shown to be due to an increase in the rate of synthesis of the rRNA species derived from the 35S precursor. The rate of synthesis of 5S rRNA and of tRNA increased much later and to a lesser extent than that of the 35S derived species. The implications of these results for general theories of regulation of RNA synthesis are discussed.
一项关于在铵抑制条件下生长的酵母原养型菌株中，添加酪蛋白氨基酸进行上调处理所产生影响的研究。在向培养基中添加氨基酸约120分钟后，上调处理使生长速率增加。这种生长速率的增加略先于DNA积累速率的增加。相比之下，蛋白质积累速率立即增加，而RNA积累速率在处理后15 - 20分钟增加。RNA最初的积累速率高于维持新稳态所需的速率。这表明这是由于源自35S前体的rRNA种类的合成速率增加所致。5S rRNA和tRNA的合成速率比源自35S的种类增加得晚得多且程度较小。讨论了这些结果对RNA合成调控一般理论的意义。
