鼠伤寒沙门氏菌和大肠杆菌色氨酸合成酶A亚基的核苷酸序列：进化比较
Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison.
作者信息
Nichols B P, Yanofsky C
出版信息
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5244-8. doi: 10.1073/pnas.76.10.5244.
Abstract
The complete nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli were determined. The nucleotide sequences are 24.8% divergent, compared with amino acid sequence divergence of 14.9%. Over half of the codons of each gene contain synonymous nucleotide changes. The pattern of synonymous nucleotide changes is consistent with the interpretation that such changes result from random mutational events. We do not find any evidence indicating that codon selection or RNA structure is of major selective value. We conclude that polypeptide function is the primary basis of selection in trpA and that most synonymous codon changes are selectively neutral.
摘要
测定了鼠伤寒沙门氏菌和大肠杆菌trpA的完整核苷酸序列。核苷酸序列的差异为24.8%，而氨基酸序列的差异为14.9%。每个基因超过一半的密码子含有同义核苷酸变化。同义核苷酸变化的模式与这种变化是由随机突变事件导致的解释一致。我们没有发现任何证据表明密码子选择或RNA结构具有主要的选择价值。我们得出结论，多肽功能是trpA选择的主要基础，并且大多数同义密码子变化是选择性中性的。
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本文引用的文献
1
Primary sequence of glutamic acid tRNA II from Escherichia coli.来自大肠杆菌的谷氨酸tRNA II的一级序列。
FEBS Lett. 1972 Feb 1;20(2):239-241. doi: 10.1016/0014-5793(72)80804-4.
2
Non-Darwinian evolution.非达尔文式进化
Science. 1969 May 16;164(3881):788-98. doi: 10.1126/science.164.3881.788.
3
Evolutionary rate at the molecular level.分子水平的进化速率。
Nature. 1968 Feb 17;217(5129):624-6. doi: 10.1038/217624a0.
4
Comparison of the tryptophan synthetase alpha-subunits of several species of Enterobacteriaceae.几种肠杆菌科细菌色氨酸合成酶α亚基的比较。
J Bacteriol. 1966 May;91(5):1819-26. doi: 10.1128/jb.91.5.1819-1826.1966.
5
Extent of variation in three related bacteriophage RNA molecules.三种相关噬菌体RNA分子的变异程度。
J Mol Biol. 1972 Jul 28;68(3):417-28. doi: 10.1016/0022-2836(72)90096-4.
6
Primary sequence of tRNA-Val-1 from Escherichia coli B. II. Isolation of large fragments by limited digestion with RNases, and overlapping of fragments to reduce the total primary sequence.来自大肠杆菌B的tRNA-Val-1的一级序列。II. 用核糖核酸酶有限消化分离大片段，并使片段重叠以缩减总一级序列。
Biochemistry. 1971 Aug 17;10(17):3277-83. doi: 10.1021/bi00793a018.
7
The sequence of nucleotides in tRNA Ile from E. coli B.来自大肠杆菌B的异亮氨酸转运RNA（tRNA Ile）中的核苷酸序列。
Biochem Biophys Res Commun. 1971 May 21;43(4):729-34. doi: 10.1016/0006-291x(71)90676-0.
8
9
Immunochemical and enzymatic comparisons of the tryptophan synthase alpha subunits from five species of Enterobacteriaceae.五种肠杆菌科细菌色氨酸合成酶α亚基的免疫化学和酶学比较
J Bacteriol. 1969 Mar;97(3):1310-20. doi: 10.1128/jb.97.3.1310-1320.1969.
10
The amino acid sequence of the A protein (alpha subunit) of the tryptophan synthetase of Escherichia coli.大肠杆菌色氨酸合成酶A蛋白（α亚基）的氨基酸序列。
J Biol Chem. 1967 Nov 25;242(22):5442-6.
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