产气克雷伯菌、鼠伤寒沙门氏菌和大肠杆菌的trpA核苷序列与trp操纵子紧邻区域序列的比较。
Comparison of the nucleoside sequence of trpA and sequences immediately beyond the trp operon of Klebsiella aerogenes. Salmonella typhimurium and Escherichia coli.
作者信息
Nichols B P, Blumenberg M, Yanofsky C
出版信息
Nucleic Acids Res. 1981 Apr 10;9(7):1743-55. doi: 10.1093/nar/9.7.1743.
Abstract
The nucleotide sequence of trpA of Klebsiella aerogenes is presented and compared with the trpA sequences of Salmonella typhimurium and Escherichia coli. The majority of the approximately 200 differences between each pair of trpA's are single nucleotide pair changes that do not alter the amino acid sequence. Codon usage conforms to the general patterns revealed by examination of other prokaryotic gene sequences. However, codon usage in K. aerogenes trpA reflects the high G+C content of the genome of this organism. The DNA sequences just beyond trpA, the presumed transcription termination region, are also compared for the three species. Perusal of these sequences indicates that the secondary structure of the transcript segment just beyond trpA has been preserved, while the primary sequence has diverged appreciably.
摘要
本文给出了产气克雷伯菌色氨酸合成酶A（trpA）的核苷酸序列，并将其与鼠伤寒沙门氏菌和大肠杆菌的trpA序列进行了比较。每对trpA之间约200个差异中的大多数是单核苷酸对变化，这些变化不会改变氨基酸序列。密码子使用符合通过检查其他原核基因序列所揭示的一般模式。然而，产气克雷伯菌trpA中的密码子使用反映了该生物体基因组的高G+C含量。还对这三个物种trpA之后假定的转录终止区域的DNA序列进行了比较。对这些序列的研读表明，trpA之后转录本片段的二级结构得以保留，而一级序列则有明显分歧。
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