School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding 071002, China; Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding 071002, China.
Sci Total Environ. 2024 Sep 20;944:173798. doi: 10.1016/j.scitotenv.2024.173798. Epub 2024 Jun 4.
Trehalase gene is mainly expressed in the digestive circulatory system for regulating energy metabolism and chitin synthesis in insects, but it is significantly expressed in gill for immunomodulation in shrimp. However, its function in regulating immunity, particularly metal resistance in crustaceans has yet to be elucidated. In this study, one Tre2 gene (NdTre2) was isolated from Neocaridina denticulata sinensis. It could bind to Cd and inhibit its toxicity. Spatiotemporal expression analysis showed that the expression of NdTre2 was highest in the gill and significantly reduced at 12 h after Cd stimulation. The transcriptomic analysis of the gill after NdTre2 knockdown showed that the expression of genes synthetizing 20E was up-regulated and the increased 20E could further induce apoptosis by activating the intrinsic mitochondrial pathway, exogenous death receptor-ligand pathway, and MAPK pathway. In vitro, overexpressing NdTre2 enhanced the tolerance of E. coli in Cd environment. In summary, these results indicate that NdTre2 plays an essential role in regulating immunity and chitin metabolism in N. denticulata sinensis.
海藻糖酶基因主要在昆虫的消化循环系统中表达，用于调节能量代谢和几丁质合成，但在虾类的鳃中则显著表达，用于免疫调节。然而，其在调节免疫，特别是甲壳类动物的金属抗性方面的功能尚未阐明。本研究从秀丽白虾中分离出一个海藻糖酶基因（NdTre2）。该基因能够与 Cd 结合并抑制其毒性。时空表达分析表明，NdTre2 在鳃中的表达最高，在 Cd 刺激 12 小时后显著降低。NdTre2 敲低后鳃的转录组分析表明，合成 20E 的基因表达上调，增加的 20E 可以通过激活内在线粒体途径、外源性死亡受体配体途径和 MAPK 途径进一步诱导细胞凋亡。在体外，过表达 NdTre2 增强了秀丽白虾对 Cd 环境中大肠杆菌的耐受性。综上所述，这些结果表明 NdTre2 在调节秀丽白虾的免疫和几丁质代谢方面发挥着重要作用。
