Ogasawara M, Kobayashi S, Hill J L, Kono D H, Yu D T
Immunology. 1985 Apr;54(4):665-76.
Three strains of bacteria which induce reactive arthritis were collected: a Shigella flexneri, designated 7060; another Sh. flexneri, designated 316; and a Yersinia enterocolitica of serotype 03. Rabbit antisera were generated against each of them to test for the extent and nature of cross-reactivity among these strains. When analysed by the ELISA technique, antisera against 7060 and 316 showed strong cross-reactivity with Y. enterocolitica. In contrast, the reaction of antisera prepared against putatively non-arthritis-causing bacteria reacted several-folds less. Using immunoprecipitation, sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western Blot procedures, a 92,000 MW cross-reactive antigen on the Yersinia was identified. The antigen was present on the outer membranes of the Y. enterocolitica, and enzyme digestion experiments showed that this antigen was protein in nature.
一株福氏志贺菌，编号为7060；另一株福氏志贺菌，编号为316；以及一株03血清型小肠结肠炎耶尔森菌。针对每一种菌株制备兔抗血清，以检测这些菌株之间交叉反应的程度和性质。通过酶联免疫吸附测定（ELISA）技术分析时，针对7060和316的抗血清与小肠结肠炎耶尔森菌表现出强烈的交叉反应。相比之下，针对假定不会引起关节炎的细菌制备的抗血清反应则小几倍。使用免疫沉淀、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质免疫印迹法，在耶尔森菌上鉴定出一种分子量为92,000的交叉反应性抗原。该抗原存在于小肠结肠炎耶尔森菌的外膜上，酶消化实验表明这种抗原本质上是蛋白质。
