Lahesmaa-Rantala R, Ståhlberg T H, Granfors K, Kuusisto P, Toivanen A
Department of Medical Microbiology, Turku University, Finland.
Clin Exp Rheumatol. 1990 Jan-Feb;8(1):5-9.
In order to investigate arthritis-triggering, serologically cross-reactive antigens, sera from patients infected with arthritis-associated microbes, Salmonellae, Chlamydia trachomatis, and Sindbis-like alphavirus, were reacted against SDS-PAGE separated and immunoblotted Yersinia enterocolitica 0:3 antigens. These sera reacted with Yersinia to the same extent as did the control sera taken from patients with streptococcal, staphylococcal and Bordetella pertussis infections or from healthy blood donors. Moreover, the various sera produced different reactivity patterns, directed against several different antigens. Although sera from test subjects, as well as from controls including healthy individuals, recognized some Yersinia antigens, these patterns differed markedly from those recognized by sera taken from patients with Yersinia infection. Significantly, analysis of the reactivities against the different molecular weight antigen components of Yersinia revealed no dominant band or pattern which could thus have been defined as arthritis-associated.
为了研究引发关节炎的血清学交叉反应抗原，将感染了与关节炎相关微生物（沙门氏菌、沙眼衣原体和辛德毕斯样甲病毒）的患者血清与经SDS - PAGE分离并免疫印迹的小肠结肠炎耶尔森菌0:3抗原进行反应。这些血清与耶尔森菌的反应程度与取自链球菌、葡萄球菌和百日咳博德特氏菌感染患者或健康献血者的对照血清相同。此外，各种血清产生了针对几种不同抗原的不同反应模式。尽管受试对象的血清以及包括健康个体在内的对照血清识别出了一些耶尔森菌抗原，但这些模式与取自耶尔森菌感染患者的血清所识别的模式明显不同。值得注意的是，对针对耶尔森菌不同分子量抗原成分的反应性分析显示，没有可被定义为与关节炎相关的主导条带或模式。
