Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, Hubei, China.
Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, Hubei, China..
Trends Genet. 2024 Sep;40(9):797-809. doi: 10.1016/j.tig.2024.05.005. Epub 2024 Jun 5.
Extracellular vesicles (EVs), emerging as novel mediators between intercellular communication, encapsulate distinct bioactive cargoes to modulate multiple biological events, such as epigenetic remodeling. In essence, EVs and epigenomic profiles are tightly linked and reciprocally regulated. Epigenetic factors, including histone and DNA modifications, noncoding RNAs, and protein post-translational modifications (PTMs) dynamically regulate EV biogenesis to contribute to EV heterogeneity. Alternatively, EVs actively modify DNA, RNA, and histone profiles in recipient cells by delivering RNA and protein cargoes for downstream epigenetic enzyme regulation. Moreover, EVs display great potential as diagnostic markers and drug-delivery vehicles for therapeutic applications. The combination of parental cell epigenomic modification with single EV characterization would be a promising strategy for EV engineering to enhance the epidrug loading efficacy and accuracy.
细胞外囊泡 (EVs) 作为细胞间通讯的新型介质，可包裹不同的生物活性物质，调节多种生物学事件，如表观遗传重塑。从本质上讲，EVs 和表观基因组图谱紧密相关且相互调节。表观遗传因子，包括组蛋白和 DNA 修饰、非编码 RNA 和蛋白质翻译后修饰 (PTMs)，可动态调节 EV 的发生，有助于产生 EV 异质性。相反，EVs 通过传递 RNA 和蛋白质货物，在靶细胞中积极修饰 DNA、RNA 和组蛋白图谱，从而对下游的表观遗传酶进行调节。此外，EVs 具有作为诊断标记物和治疗应用的药物传递载体的巨大潜力。将亲本细胞表观遗传修饰与单个 EV 特征相结合，可能是增强表皮药物装载功效和准确性的 EV 工程的一种有前途的策略。
