Mi Zhipeng, Wen Wulin, Guo Fan, Shi Qiangyou, Long Chao, Zhou Yu, Ma Ruixia
The Second Clinical Medical College, Ningxia Medical University, 750000 Yinchuan, Ningxia, China.
Yinchuan First People's Hospital, Otolaryngology Head and Neck Surgery Hospital, 750000 Yinchuan, Ningxia, China.
Heliyon. 2024 May 25;10(11):e31969. doi: 10.1016/j.heliyon.2024.e31969. eCollection 2024 Jun 15.
Circular RNAs (circRNAs) significantly influence the invasion, metastasis, gene expression, proliferation, and apoptosis of tumor cells. However, the roles of circRNAs in laryngeal squamous cell carcinoma (LSCC) remain largely unexplored. This study aims to examine circRNA expression patterns in LSCC and adjacent non-tumorous tissues, with the goal of uncovering potential biomarkers for LSCC.
Tissue samples were collected from both the tumor and adjacent normal tissues of ten patients who had undergone surgical resection. The profiling of circRNAs was conducted through transcriptomic sequencing and analytical bioinformatics approaches. A ternary regulatory network based on the competitive endogenous RNA (ceRNA) hypothesis was established, linking target circRNAs to clinical immunohistochemical parameters for comparison. Verification of target circRNAs in LSCC tissues was performed using quantitative real-time PCR (RT-qPCR), whereas target mRNAs were analyzed through immunohistochemistry.
A total of 126 significantly different circRNAs were identified, including 40 up-regulated genes and 86 down-regulated genes. Furthermore, 92 circRNA-miRNA-mRNA regulatory relationship axes related to clinical immunohistochemical indicators were found based on 5 candidate circRNAs. Interestingly, all axes related to the target genes MKI67 and TP53 were found to compete with the same circRNA: hsa_circ_0069,399. Further verification confirmed that the hsa_circ_0069,399 expression was overtly upregulated in tumor tissues from LSCC patients, which was consistent with the sequencing results.
hsa_circ_0069,399 could be a potential prognostic marker for LSCC.
环状RNA（circRNAs）对肿瘤细胞的侵袭、转移、基因表达、增殖和凋亡有显著影响。然而，circRNAs在喉鳞状细胞癌（LSCC）中的作用仍 largely未被探索。本研究旨在检测LSCC及癌旁非肿瘤组织中circRNA的表达模式，以发现LSCC潜在的生物标志物。
收集10例接受手术切除患者的肿瘤组织和癌旁正常组织样本。通过转录组测序和分析生物信息学方法对circRNAs进行分析。基于竞争性内源性RNA（ceRNA）假说建立三元调控网络，将靶circRNAs与临床免疫组化参数进行关联比较。采用定量实时PCR（RT-qPCR）验证LSCC组织中靶circRNAs，通过免疫组化分析靶mRNA。
共鉴定出126个差异显著的circRNAs，包括40个上调基因和86个下调基因。此外，基于5个候选circRNAs发现了92个与临床免疫组化指标相关的circRNA-miRNA-mRNA调控关系轴。有趣的是，发现所有与靶基因MKI67和TP53相关的轴均与同一circRNA：hsa_circ_0069,399竞争。进一步验证证实，hsa_circ_0069,399在LSCC患者肿瘤组织中的表达明显上调，与测序结果一致。
hsa_circ_0069,399可能是LSCC的一个潜在预后标志物。
